FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147355
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC4344029.4798276271589No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC1510410.250076346238675No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC133949.089613518373994No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC61884.199382443758271No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC38112.5862712497030977No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC31842.1607682128193817No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC22361.517423908248787No Hit
TTACCGCGGCTGCTGGCACTGAGCCAGGATCAAACTCTTGTAGAACCATG13440.9120830647076787No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC13060.8862950018662413No Hit
TNACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC12290.8340402429506973No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC11300.7668555529164264No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC11030.7485324556343524No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCACGCTACC9630.653523803060636No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC9450.6413084048725866No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC9230.6263784737538598No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC8670.5883750127243731No Hit
TTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC7080.4804723287299379No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTACTCTTTGGGTACC5620.381391876760205No Hit
TTACCGCGGCTGCTGGCACACTGAGCCAGGATCAAACTCTTGTAGAACCA5230.3549251806860982No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC5140.34881748159207354No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC5100.34610294866139596No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC4800.3257439516813138No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTCTTCTTTGGGTACC4500.3053849547012317No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC4420.2999558888398765No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTCGGTACC3790.25720199518170406No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT3780.25652336194903463No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTTGGGTACC3750.2544874622510264No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTCAGGTACC3750.2544874622510264No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTTTGGGTACC3610.24498659699365477No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC3500.23752163143429136No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC3480.23616436496895254No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC3280.22259170031556447No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTAAC3190.21648400122153982No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2960.20087543687014353No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTAGGTACC2760.18730277221675545No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCGAGTAAC2720.18458823928607784No Hit
TTACCGCGGCTGCTGGCACGCAGTTAGCCGGTGCTTATTCTTTGGGTACC2680.18187370635540023No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC2360.1601574429099793No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC2240.15201384411794647No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGCGCTTATTCTTTGGGTACC1940.13165484713786435No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTCTGGGTACC1810.1228326151131621No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1750.11876081571714568No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTTCAGGTACC1740.11808218248447627No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1710.11604628278646806No Hit
TTACCGCGGCTGCTGGCATGAAGTTAGCCGGTGCTTATTCTTTGGGTACC1700.11536764955379865No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTCGGGTACC1670.11333174985579043No Hit
TTACCGCGGCTGCTGGCACGAGGTTAGCCGGTGCTTATTCTTTGGGTACC1650.11197448339045164No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCCTATTCTTTGGGTACC1630.11061721692511282No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCCTTGGGTACC1570.1065454175290964No Hit
TACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACCG1560.10586678429642699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)