FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202147
Sequences flagged as poor quality0
Sequence length300
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC2697813.34573355033713No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC152997.568254784884268No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC138136.8331461758027565No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC115355.706243476282111No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC106095.248160991753526No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC76103.7645871568709897No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC50532.4996660845820124No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC33521.6581992312525045No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC28511.410359787679263No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCTCCTACC20511.0146081811750856No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC18540.9171543480734318No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC13830.6841555897440972No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC11330.5604832127115416No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC10930.5406956323863327No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC10730.5308018422237283No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC10020.4956788871464825No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC9550.472428480264362No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTACTTCTTCTGCAGGTACC9420.4659975166586692No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC8820.43631614617085585No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC8200.4056453966667821No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTCCTTCTTCTGCAGGTACC8200.4056453966667821No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC7750.38338436880092214No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC7560.3739852681464479No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCAGGTACC7150.3537029983131088No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC6220.30769687405699814No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC6070.3002765314350448No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC6030.2982977734025239No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT5990.29631901537000305No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGTACCTACC5450.269605781930971No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCACGCTACC4910.24289254849193903No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATAAGATACC4650.23003062128055327No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTTTCTTATGGTACC4580.22656779472364172No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCATGTGGTACC4490.22211558915046972No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGCCTTCCTCCGTGGGTACC4390.21716869406916747No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTCCAGGTACC4300.21271648849599548No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGTTACC4270.2112324199716048No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTACAGTACC4250.21024304095534438No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC4250.21024304095534438No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGATTAGGTACC4220.20875897243095373No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC4190.20727490390656306No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC3970.19639173472769814No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3940.1949076662033075No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCCACTACC3660.18105635997566127No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCGCCTACC3620.1790776019431404No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCCAGGTACC3470.17165725932118706No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGTAGGTACC3340.16522629571549416No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC3120.1543431265366293No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCACCTACC3120.1543431265366293No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTCGGTACC3080.1523643685041084No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC3030.14989092096345727No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTATTCTCCCGGTACA2870.14197588883337375No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGTAGGTACC2840.14049182030898308No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCGCATACC2800.13851306227646218No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC2760.13653430424394128No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC2740.13554492522768086No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCCTCCTCAAGTACC2730.13505023571955063No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTGTGGGTACC2690.13307147768702976No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAAGTACC2610.12911396162198796No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGTCCTTATTCCTATGGTACT2570.1271352035894671No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC2570.1271352035894671No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC2560.12664051408133686No Hit
TTACCGCGGCTGCTGGCACGAATTTAGCCGGGGCTTATTCTGATGGTACA2440.12070423998377419No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTTCAGGTACC2410.11922017145938352No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC2400.11872548195125329No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACC2370.11724141342686263No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGACCTTATTCGTATAGTACT2320.11476796588621152No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGACTTCTTCTGCTGGTACC2310.1142732763780813No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGCCTTCCTCCATGGGTACC2200.10883169178864886No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTGAGGTACC2170.10734762326425819No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTACCACTACC2150.10635824424799774No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTTTCTGCAGGTACC2110.10437948621547687No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTGCAGGTACC2110.10437948621547687No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGATTAGGTACC2100.10388479670734663No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCATATAGTACC2070.10240072818295597No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATACAGGTACC2050.10141134916669553No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC2040.1009166596585653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)