FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112376
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC2947526.22891008756318No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1474213.118459457535417No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC47084.189506656225529No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC25282.2495906599273865No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAATTGATTACC24632.1917491279276713No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC22752.0244536199900334No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC19581.7423649177760376No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCGAGTAAC16561.4736242614081299No Hit
TTACCGCGGCTGCTGGCACACTGAGCCAGGATCAAACTCTTGTAGAACCA14721.309888232362782No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC13031.1595002491635225No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGAGCTTATTCTCCCGGTACA10320.9183455542108636No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC9840.8756318075033815No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC8830.7857549654730548No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC8680.7724069196269666No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC8000.7118957784580338No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC7700.6851996867658575No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGATTAGGTACC5070.4511639495977789No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC4960.4413753826439809No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC4940.4395956431978358No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC4700.41823876984409486No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTAAGATACC4440.39510215704420876No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC4140.36840606535203246No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC3730.33192140670605824No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTCGGTACC3440.3061151847369545No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCTGTCCCTTTCTGGTAAGATACC3430.305225315013882No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAATTACC2640.23492560689115116No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGATTAGGTACC2630.2340357371680786No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGACTAGGTACC2500.22246743076813555No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAATTGATTACC2220.19755107852210438No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC2150.19132199046059659No Hit
TTACCGCGGCTGCTGGCACGAACTTAGCCCGTGCTTCCTCCAGGGATCAG2070.18420303267601623No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTCTGATTAGGTACC1990.1770840748914359No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC1960.17441446572221828No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTTTTCTGCAGGTACC1940.17263472627607318No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTAAC1880.16729550793763792No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1870.1664056382145654No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC1850.16462589876842032No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGACCCTTATTCATGAGTTACC1810.16106641987613013No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCGGCTTTCTGATTAGGTACC1800.1601765501530576No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC1630.14504876486082438No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCCGATTAGGTACC1590.14148928596853422No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGCTTAGGTACC1560.1388196767993166No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTTTGATTAGGTACC1560.1388196767993166No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCGAGTAAC1550.13792980707624403No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTCTCTGATTAGGTACC1510.13437032818395386No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC1420.126361500676301No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGTTTAGGTACC1350.12013241261479318No Hit
TACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACCG1340.11924254289172065No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCCTTCTGATTAGGTACC1320.11746280344557558No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGGATACC1310.11657293372250303No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATCAGGTACC1290.11479319427635795No Hit
CTACCGCGGCTGCTGGCACACTGAGCCAGGATCAAACTCTTGTAGAACCA1280.1139033245532854No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC1260.11212358510714032No Hit
TTACCGCGGCTGCTGGCCCGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1220.10856410621485015No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTGATTAGGTACC1210.1076742364917776No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGTGGCTTTCTGATTAGGTACC1200.10678436676870506No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTCATTGATTACC1170.10411475759948743No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1160.10322488787641489No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC1150.10233501815334234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)