FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141263
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG4612132.649030531703275No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG3626825.674097251226435No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG82355.829551970438119No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAACACATG76745.432420379009365No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGGGCCTCATACATG69654.930519668986217No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCGTGCTTTACACATG27131.9205312077472516No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGGATAAGGCATG21771.5410971025675513No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG10570.7482497186099686No Hit
AGAGTTTGATCCTGGCTCAGAATCAACGCTGGCGGCGCGCTTAACACATG5170.3659840156304198No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG5130.36315241783057134No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG5110.3617366189306471No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG3980.28174398108492665No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG3110.22015672893822164No Hit
AGAGTTTGATCCTGGCTCGGGATGAACGCTGGCGGCGTGCCTAATACATG3080.21803303058833523No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG2980.21095403608871396No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2920.20670663938894118No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2900.20529084048901697No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG2260.15998527569144078No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG2030.14370358834231187No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG1980.14016409109250122No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATG1980.14016409109250122No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG1920.13591669439272847No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACACG1920.13591669439272847No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGGGCCTAATACATG1870.13237719714291782No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG1860.13166929769295568No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTCATACATG1740.12317450429341016No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTCATACATG1710.12105080594352378No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG1650.11680340924375102No Hit
AGAGTTTGATCCTGGCTCAGGATGAGCGCTGGCGGCGTGCCTAATACATG1630.11538761034382676No Hit
AGAGTTTGATCCTGGCTCAGCCACTTCATGTGTGTGTCTTAGGCCAGTTA1530.10830861584420548No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACGTG1520.10760071639424336No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG1510.10689281694428124No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCGTGCCTAATACATG1500.10618491749431912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)