FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141263
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3767226.667988078973266No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC2328516.483438692368136No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTGCGGGTACC64244.5475460665567065No Hit
TTACCGCGGCTGCTGGCACAGAGTTTGCCGGTGCTTCCTCTCCTGGTACC56864.025116272484657No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC50543.5777238201085915No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC41852.962559198091503No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC26321.8631913523003192No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC23281.6479899195118326No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC23091.6345398299625522No Hit
TTACCGCGGCTGCTGGCACGAGATTTGCAGGCCCTTATTCGGCAGGTACC16581.1736972880372072No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC15301.0830861584420548No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC10450.7397549252104232No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTTTTCTGCAGGTACC7560.5351719841713682No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC6490.45942674302542064No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC4290.30368886403375267No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTGCGGGTACC4280.30298096458379054No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCAGCTTCTTCTCCGGGTACC4250.30085726623390413No Hit
CTACCGCGGCTGCTGGCACAGAGTTTGCCGGTGCTTCCTCTCCTGGTACC4050.2866992772346616No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC3730.2640464948358735No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC3430.2428095113370097No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGCAAGCTACC3140.22228042728810798No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC3130.22157252783814585No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC3040.21520143278848672No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2930.20741453883890332No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2680.18971705258985014No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC2610.18476175644011525No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC2370.16777216964102418No Hit
TNACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTGCGGGTACC2090.14795098504208462No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGCTACC1950.13804039274261484No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC1870.13237719714291782No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTCCTTTCTGGTAAGATACC1780.12600610209325866No Hit
TNACCGCGGCTGCTGGCACAGAGTTTGCCGGTGCTTCCTCTCCTGGTACC1670.11821920814367526No Hit
TTACCGCGGCTGCTGGCCCGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1660.11751130869371315No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCCCTTTCTGGTAAGATACC1620.11467971089386463No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCCGGTAAGATACC1620.11467971089386463No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATAAC1600.11326391199394038No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC1530.10830861584420548No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTCCTGGTAAGATACC1530.10830861584420548No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGTTAAGATACC1510.10689281694428124No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTCTCTGGTAAGATACC1500.10618491749431912No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTCCTCTCCTGGTACC1470.10406121914443273No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC1450.10264542024450848No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCCTTCTGGTAAGATACC1430.10122962134458421No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGCTACC1420.10052172189462208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)