FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143544
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG4008627.925932118374853No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1980313.795769938137436No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1609611.213286518419439No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG1480210.311820765758235No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG105757.367079083765257No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG31852.218831856434264No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG20441.423953630942429No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG20321.4155938248899291No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCCTAACACATG7880.5489605974474726No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG7160.49880176113247504No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG7160.49880176113247504No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG6730.46884578944435157No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAACACATG6320.44028311876497794No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG6070.4228668561556038No Hit
AGAGTTTGATCCTGGCTCAGGATGAATGCTGGCGGCGTGCCTAACACATG5950.4145070501031043No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCATAACACATG5520.3845510784149808No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGTATGCTTAACACATG4860.33857214512623307No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCTTAACACATG4570.3183692804993591No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG4140.28841330881123556No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG3940.2744802987237363No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG3570.24870423006186254No Hit
AGAGTTTGATCCTGGCTCAGGGTGAACGCTAGCGGCGTGCCTTAAGCATG3160.22014155938248897No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAACACATG3130.2180516078693641No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG3100.2159616563562392No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG3060.21317505433873932No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG2650.18461238365936578No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG2400.16719612104999163No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGTGTGCCTAATACATG2300.160229616006242No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACACATG2030.14142005238811794No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTGACACATG1920.1337568968399933No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG1810.1260937412918687No Hit
AGAGTTTGATCCTGGCTCGGGATGAACGCTGGCGGCGTGCCTAATACATG1650.11494733322186924No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG1640.11425068271749428No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG1620.11285738170874436No Hit
AGAGTTTGATCCTGGCTCAGCTGGGTCCTGTCCGGCTCTGACACATGCCC1540.10728417767374464No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG1510.10519422616061974No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG1450.10101432313436995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)