FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143544
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1856612.934013264225602No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC141109.829738616730758No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC124958.70464805216519No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC75015.225575433316614No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT54783.816251462966059No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC51923.617009418714819No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT28661.9966003455386503No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC26661.857270244663657No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC23501.6371286852811682No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC18081.259544111909937No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC16021.1160341080086942No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT15301.0658752716936968No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC13750.9578944435155772No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC13690.9537145404893274No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC10930.761439001281837No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC10780.7509892437162125No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC9440.6576380761299672No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC8460.5893663267012206No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC8010.5580170540043471No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCTCCTACC7740.539207490386223No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC6740.4695424399487265No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC6450.44933957532185254No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTCTTTTAGGGTACT5990.41729365212060415No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCTTACGGTACA5850.40754054505935455No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC5830.40614724405060465No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC5550.3866410299281057No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC5380.3747979713537313No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC4960.3455386501699827No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT4930.34344869865685784No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCATGAGTTACC4910.3420553976481079No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGACCTTATTCGTATAGTACT4820.3357855431087332No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCGAGTAAC4810.33508889260435826No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC4350.3030429694031099No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGCGCTTCTTCTGTAGGTACC4290.29886306637686005No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTGATTCCTTAGGTACC3900.27169369670623644No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC3890.2709970462018615No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCTAGGTACC3690.2570640361143621No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC3660.25497408460123727No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTTACGATACC3490.24313102602686287No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTGTGGGTACC3410.23755782199186312No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCCCCCACTACC3290.22919801593936354No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAAGTACC3210.22362481190436384No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTTAGTAGGTACC3150.21944490887811402No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTACC2630.18321908265061584No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTTTTCTGCAGGTACC2590.18043248063311598No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT2480.17276932508499135No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC2450.17067937357186647No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCAGGTGCTTATTCTCCAAGTCTC2330.16231956751936688No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC2310.16092626651061695No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC2240.1560497129799922No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCCGTTACC2080.14490330490999276No Hit
TNACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2060.14351000390124283No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC1960.13654349885749317No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC1840.1281836928049936No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC1830.12748704230061864No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGAATACC1810.1260937412918687No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATCAGGTACC1800.12539709078749373No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCACCTACC1720.119823886752494No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGATTAGGTACC1710.11912723624811904No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCCGGTACT1620.11285738170874436No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC1610.1121607312043694No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC1610.1121607312043694No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC1590.11076743019561945No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCCTCCCATGGTACC1590.11076743019561945No Hit
TTACCGCGGCTGCTGGCACTCAGGTTTCTGTCCCCTTCTCCCAGGTCACG1530.10658752716936966No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGCGCTTCTTCTGCAGGTACC1520.1058908766649947No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC1490.10380092515186981No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC1480.10310427464749485No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT1470.1024076241431199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)