FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157331
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG6055538.48891826785567No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG2729217.34686743235599No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1744211.086181362859195No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG94426.00136018966383No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG39662.520800096611602No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG26451.6811690003877178No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG14080.8949285264823843No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG7580.48178680616026087No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCCTAACACATG6790.43157419707495664No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG6660.42331136266851416No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCTTAACACATG3850.24470701896002695No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG3800.24152900572677988No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG3530.2243677342672455No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCGGGCCTAACACATG3380.2148336945675042No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG3290.20911327074765942No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG3280.20847766810100998No Hit
AGAGTTTGATCCTGGCTCAGGTTGAACGCTGGCGGCAGGCCTAACACATG3020.19195199928812504No Hit
AGAGTTTGATCCTGGCTCAGATCGAACGCTGGCGGCAGGCCTAACACATG2930.18623157546828026No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAATACATG2670.1697059066553953No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG2480.15762945636905631No Hit
AGAGTTTGATCCTGGCTCGGATTGAACGCTGGCGGCAGGCCTAACACATG2380.1512734299025621No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAACACATG2370.1506378272559127No Hit
AGAGTTTGATCCTGGCTCAGATTGAGCGCTGGCGGCAGGCCTAACACATG2280.1449174034360679No Hit
AGAGTTTGATCCTGGCTCAGACTGAACGCTGGCGGCAGGCCTAACACATG2270.1442818007894185No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCAGGCCTAACACATG2270.1442818007894185No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACACG2250.14301059549611964No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2240.14237499284947022No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG2070.13156974785643008No Hit
AGAGTTTGATCCTGGCTCAGATTGGACGCTGGCGGCAGGCCTAACACATG2040.1296629399164818No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCCGGCGGCAGGCCTAACACATG1990.12648492668323472No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCGTGCCTAATACATG1910.12140010551003934No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACGCATG1890.12012890021674051No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACGTG1810.11504407904354515No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)