FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142524
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG6362844.64370912969044No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG2465517.298840896971736No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG136309.563301619376386No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG75015.262973253627459No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGTGTGCCTAATACATG19481.3667873480957593No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGCGCCTAACACATG7750.5437680671325531No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAACACATG7510.5269287979568353No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG5980.41957845696163454No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCAGGCTTAACACATG5430.3809884651006146No Hit
AGAGTTTGATCCTGGCTCAGGACAAACGCTGGCGGGGTGCGTGAGACATG5190.36414919592489686No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG4100.287670848418512No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGTGTGCCTAATACATG3850.27012994302713933No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCCTAACACATG3670.25750049114535095No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG3440.2413628581852881No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCTTAACACATG3410.23925794953832338No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG3300.2315399511661194No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG3270.22943504251915467No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG3200.2245235890095703No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG3100.21750722685302126No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG2840.19926468524599367No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG2630.1845303247172406No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG2590.181723779854621No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG2460.1726025090511072No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG2410.16909432797283266No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG2110.14804524150318543No Hit
AGAGTTTGATCCTGGCTCAGGACGGACGCTGGCGGCGTGCCTAATACATG1920.1347141534057422No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG1900.1333108809744324No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAATACATG1860.13050433611181275No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1830.12839942746484803No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAGTACATG1830.12839942746484803No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGGGCTTAACACATG1690.11857652044567932No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGGGCCTAATACATG1660.1164716117987146No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTCATACATG1640.11506833936740478No Hit
AGAGTTTGATCCTGGCTCAGGACGCACGCTGGCGGCGTGCCTAATACATG1610.11296343072044006No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCCAATACATG1540.1080519772108557No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1500.10524543234823609No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG1500.10524543234823609No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATG1490.10454379613258119No Hit
AGAGCTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1470.10314052370127137No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTACTACATG1430.10033397883865174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)