FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142524
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC5199236.479470124329936No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC2143215.037467373915971No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC48583.408548735651539No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC42963.0142291824534815No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC32282.2648817041340403No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC28592.0059779405573797No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC20801.459403328562207No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGAATACC16361.1478768488114282No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC15101.05947068563891No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC13290.9324745306053718No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC6760.47430608178271727No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC6100.4279980915494934No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC5130.3599393786309674No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTATTCCTCAGGTACC4900.34380174567090455No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC4720.3311722937891162No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC4010.2813561224776178No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC3540.24837922034183718No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC3350.23504813224439394No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC3030.2125957733434369No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC3020.21189413712778196No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC2920.2048777749712329No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC2860.20066795767730347No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGGTAGTTACC2800.19645814038337403No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCCCTTTCTGGTAAGATACC2560.17961887120765627No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC2490.1747074176980719No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2460.1726025090511072No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCCGGTACC2450.17190087283545227No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC2430.17049760040414244No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGACCCTTATTCATAAGTTACC2350.1648845106789032No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCTGGTACC2240.15716651230669923No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATAAC2130.14944851393449526No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC2070.1452386966405658No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTCTCTGGTAAGATACC2040.14313378799360107No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAGGATACC2040.14313378799360107No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCCTTCTGGTAAGATACC1980.13892397069967163No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGTTAAGATACC1950.1368190620527069No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTCCTGGTAAGATACC1930.1354157896213971No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTTCTGGTACC1890.13260924475877747No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1860.13050433611181275No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCAGGTACC1850.12980269989615784No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTCGTAAGATACC1820.12769779124919312No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCCGGTAAGATACC1790.1255928826022284No Hit
TACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACCG1770.12418961017091859No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTCCTCTGTTACC1740.12208470152395386No Hit
TTACCGCGGCTGCTGGCCCGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1720.12068142909264405No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCCTCTGCTGGTACC1690.11857652044567932No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGCAAGATACC1620.11366506693609499No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTAAC1590.11156015828913024No Hit
TTACCGCGGCTGCCGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1540.1080519772108557No Hit
TTACCGCGGCTGCTGGCACGTAGCTAGCCGTCCCTTTCTGGTAAGATACC1460.10243888748561646No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC1450.10173725126996155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)