FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118659
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3266527.528463917612655No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC101368.542124912564576No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC74966.317262070302294No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC63075.315231040207654No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC56564.7666000893316145No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC35192.9656410386064267No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC19311.6273523289426002No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC18231.5363352126682341No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC14811.2481143444660752No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC14721.2405295847765445No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC11590.976748497796206No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC9330.7862867544813289No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC9110.7677462307958098No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTAGGTACC8470.7138101618924818No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC5910.4980658862791697No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC5620.4736261050573492No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACC5590.4710978518275057No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC5000.4213755383072502No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC4770.4019922635451167No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC3850.3244591644965827No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC3840.32361641341996816No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC3750.3160316537304377No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC3680.31013239619413613No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC3560.30001938327476213No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTACCACTACC3390.28569261497231563No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTGTTCTACAGGTACC3360.2831643617424721No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC3290.2772651042061706No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC3280.2764223531295561No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGTCCTTATTCGTATGGTACT3140.2646238380569531No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC3060.2578818294440371No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACC2980.2511398208311211No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC2850.2401840568351326No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCATAAGGTACA2510.21153052023023958No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTACAGTACC2400.2022602583874801No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC2170.18287698362534657No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC2100.17697772608904508No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC2000.1685502153229001No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC1940.16349370886321307No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1840.15506619809706806No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCCCTTTCTGGTAAGATACC1740.14663868733092306No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATAAC1700.14326768302446508No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC1690.14242493194785055No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACT1650.13905392764139257No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1600.13484017225832007No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC1540.12978366579863304No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTCTCTGGTAAGATACC1520.12809816364540405No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC1490.12556991041556054No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTCCTGGTAAGATACC1390.11714239964941554No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTATTCCGGTACC1380.11629964857280105No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTTAGGTACC1370.11545689749618655No Hit
TACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACCG1350.11377139534295756No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCCTTCTGGTAAGATACC1280.10787213780665604No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCCGGTAAGATACC1220.10281563134696904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)