FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120652
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG4313435.750754235321416No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG2229518.47876537479694No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1949816.16052779895899No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG22061.8283990319265326No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG11780.9763617677286742No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG11680.9680734674932865No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGAATGCCTAACACATG10680.8851904651394092No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAACACATG9980.8271723634916951No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG9540.790703842455989No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTCATGCATG9000.7459470211848954No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG6080.5039286543115737No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG5970.49481152405264733No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTAACACATG5630.46663130325232904No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGGATGCTTTACACATG5000.41441501176938633No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGTATGCTTAACACATG4250.3522527600039784No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG3480.2884328481914929No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG3430.28428869807379903No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG2910.24118953684978284No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG2710.2246129363790074No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG2560.21218048602592582No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG2470.20472101581407687No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG2460.20389218579053808No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2320.19228856546099526No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2180.18068494513145245No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG1900.1574777044723668No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1870.15499121440175048No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG1840.15250472433113416No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG1770.14670291416636275No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG1690.14007227397805258No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG1570.1301263136955873No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCTTAACACATG1570.1301263136955873No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAATACATG1410.11686503331896694No Hit
AGAGTTTGATCCTGGCTCAGGACGGACGCTGGCGGCGTGCCTAATACATG1370.11354971322481186No Hit
AGAGTTTGATCCTGGCTCAGCCACTTCATGTGTGTGTCTTAGGCCAGTTA1340.11106322315419553No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCTTAACACATG1270.10526141298942412No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCCAATACATG1240.10277492291880781No Hit
AGAGTTTGATCCTGGCTCAGGATGAATGCTGGCGGCGTGCCTTAGGCATG1230.10194609289526904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)