FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120652
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3069025.436793422404936No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC103178.551039352849518No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC79176.561847296356463No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC47953.974239962868415No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC29922.4798594304280077No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC26982.2361834035076087No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC24772.05301196830554No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTGTGGGTACC18911.567317574511819No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCATAAGTTACC10540.8735868448098663No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTTTTCTGCAGGTACC10050.8329741736564665No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCATAAGGTACA9610.7965056526207606No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC9250.7666677717733648No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCTCCTACC9050.7500911713025893No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTGAGGTACC8860.7343434008553527No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC8750.7252262705964261No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC8130.6738388091370222No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC7690.6373702881013162No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCGCTTCTTCTGCAGGTACA7430.6158207074893081No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTATTCCCAAGGTACC7070.5859828266419123No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGTGGGTACA6940.5752080363359082No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC6780.5619467559592879No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTCTCCTTGGGTACC6250.5180187647117329No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC4980.4127573517223088No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGTAGGTACC4590.38043298080429666No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTGCAGGTACC4400.36468521035706No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGTGGGTAAC4340.3597122302158274No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT4030.3340184994861254No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC4030.3340184994861254No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC3830.3174418990153499No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCCTTAGGTACC3510.2909193382621092No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC3330.2760003978384113No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTGCTGGTTAGATACC3140.2602526273911746No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTTAGGTACC2870.23787421675562775No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC2640.21881112621423598No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC2580.21383814607300336No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC2580.21383814607300336No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTACAGTACC2540.21052282597884825No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC2500.20720750588469317No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC2320.19228856546099526No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC2190.18151377515499123No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC2100.17405430494314228No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC2060.17073898484898717No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC2010.1665948347312933No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGATACC1880.15582004442528927No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC1800.14918940423697907No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCCGGTAAGATACC1720.1425587640486689No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC1700.14090110400159134No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1670.13841461393097504No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTATTCCGGTACC1580.1309551437191261No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC1520.12598216357789344No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCCCTTTCTGGTAAGATACC1490.12349567350727712No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTGTGGGTACC1430.11852269336604448No Hit
TTACCGCGGCTGCTGGCCCGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1410.11686503331896694No Hit
TTACCGCGGCTGCTGGCACGTAGCTAGCCGTCCCTTTCTGGTAAGATACC1320.10940556310711799No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTCTCTGGTAAGATACC1320.10940556310711799No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTCCTGGTAAGATACC1260.10443258296588535No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCCTTCTGGTAAGATACC1260.10443258296588535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)