FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160154
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG5470234.155874970341046No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG4833530.180326435805537No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG2081212.994992319892104No Hit
AGAGTTTGATCCTGGCTCAGTGTGTGCCAGCAGCCGCGGTAAAGACCAAG14760.9216129475379947No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG13580.8479338636562309No Hit
AGAGTTTGATCCTGGCTCAGGATGAATGCTGGCGGCGTGCCTAACACATG10470.653745769696667No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG7600.47454325211983467No Hit
AGAGTTTGATCCTGGCTCAGAATCAACGCTGGCGGCGTGCCTCAGACATG7200.44956729148194857No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG6940.4333329170673227No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG4160.25974999063401477No Hit
AGAGTTTGATCCTGGCTCGGGATGAACGCTGGCGGCGTGCCTAATACATG2990.18669530576819812No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG2790.1742073254492551No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2680.16733893627383642No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGTTAGCGGCACGCCTAACACATG2490.15547535497084056No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG2440.1523533598911048No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG2400.14985576382731622No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATG2390.14923136481136906No Hit
AGAGTTTGATCCTGGCTCAGGATGAGCGCTGGCGGCGTGCCTAATACATG2320.144860571699739No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG2270.14173857662000325No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2240.13986537957216177No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG2220.1386165815402675No Hit
AGAGTTTGATCCTGGCTCAGGATGAATGCTGGCGGCGTGTCTAAGGCATG2120.132372591380796No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACACG2080.12987499531700739No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACGTG2040.12737739925321878No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG1880.11738701499806437No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGCGTGCCTAATACATG1790.11176742385454001No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG1790.11176742385454001No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG1720.10739663074290995No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAATACATG1680.10489903467912134No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG1650.10302583763127988No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG1610.10052824156749128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)