FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148979
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC5557437.3032440813806No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT140189.409379845481578No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC51873.4816987629128935No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC43192.899066311359319No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC32632.190241577672021No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTACC22201.490142906047161No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC19241.291457185240873No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC16961.1384154813765697No Hit
TNACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC15111.0142369058726397No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC10460.7021123782546533No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT8740.5866598648131616No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTCTTCTGTCGGTAAC7850.5269199014626222No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAATTACC6350.42623456997294923No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTACTCTGTCGGTAAC6290.4222071567133623No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC6150.4128098591076595No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTAGGTAAC5800.38931661509340243No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCCGATACT4990.33494653608897895No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTCGGTAAC4700.31548070533430883No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACC4230.283932634800878No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTCAC4110.27587780828170416No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT3370.2262063780801321No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC3360.2255351425368676No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTGTCGGTAAC3340.22419267145033867No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC3260.21882278710422276No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2990.2006994274360816No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTCTCGGTAAC2630.17653494787856006No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC2490.16713765027285726No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCCGGTAAC2390.16042529484021237No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCCTATTCTGTCGGTAAC2330.15639788158062545No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGCGCTTATTCTGTCGGTAAC2250.15102799723450958No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATCCTGTCGGTAAC2110.14163069962880676No Hit
TACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAACG2100.14095946408554227No Hit
TTACCGCGGCTGCTGGCCCAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2070.1389457574557488No Hit
TTACCGCGGCTGCTGGCACAGCGTTAGCCGGTGCTTATTCTGTCGGTAAC2040.13693205082595533No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTCGGTACC2000.13424710865289738No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGAGCTTATTCTCCCGATACT2000.13424710865289738No Hit
TTACCGCCGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC1980.13290463756636842No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCCGTCGGTAAC1950.13089093093657497No Hit
TTACCGCGGCTGCTGGCACAGGGTTAGCCGGTGCTTATTCTGTCGGTAAC1930.12954845985004598No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT1890.12686351767698803No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGATACT1810.12149363333087214No Hit
TTACCGCGGCTGCTGGCACAGAGCTAGCCGGTGCTTATTCTGTCGGTAAC1740.11679498452802073No Hit
TTACCGCGGCCGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC1590.10672645137905343No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1520.10202780257620202No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAGC1520.10202780257620202No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCCGATACT1510.10135656703293752No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTCTCGGTACC1500.10068533148967304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)