FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136144
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG6180645.397520272652486No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG2237616.435538841226936No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG54804.025149841344459No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG46623.4243154307204136No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG29682.18004465859678No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG26851.9721765189799036No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG17221.2648372311669998No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGTGTGCCTAATACATG13761.0106945587025502No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTTACACATG11760.8637912798213656No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG10530.773445763309437No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG9450.6941179927135973No Hit
AGAGTTTGATCCTGGCTCAGTGCCAGCAGCCGCGGTAAAGACCAAGTCTC8020.5890821483135504No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG4730.34742625455400167No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGAATGCTTTACACATG4500.3305323774826654No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG4490.3297978610882595No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG4210.30923140204489363No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG4200.3084968856504877No Hit
AGAGTTTGATCCTGGCTCAGGATGAATGCTGGCGGCGTGCCTAACACATG3910.28719591021271595No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG3830.2813197790574685No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG3340.24532847573157834No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2950.21668233634974735No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAATACATG2830.20786813961687625No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG2630.19317781172875778No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG2470.18142554941826303No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG2470.18142554941826303No Hit
AGAGTTTGATCCTGGCTCAGGACGGACGCTGGCGGCGTGCCTAATACATG2430.1784874838406393No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG2130.15645199200846163No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG2110.15498295921964977No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCTTAACACATG2030.1491068280644024No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGGGCCTCATACATG2020.14837231166999648No Hit
AGAGTTTGATCCTGGCTCAGAAGGAACGCTAGCTATATGCTTAACACATG2010.14763779527559054No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATA1890.13882359854271947No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG1840.13515101657068984No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAATACATG1830.13441650017628393No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACACATG1810.1329474673874721No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG1660.12192972147138324No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCCAATACATG1620.11899165589375955No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAGTACATG1520.11164649194970032No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTCATACATG1520.11164649194970032No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTACTACATG1500.11017745916088847No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1440.10577036079445293No Hit
AGAGTTTGATCCTGGCTCAGGACGCACGCTGGCGGCGTGCCTAATACATG1440.10577036079445293No Hit
AGAGCTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1430.105035844400047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)