FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136144
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2109815.496826889176166No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1774113.031055353155482No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC129489.510518274767893No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAATTACC96157.062375132212952No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC32532.389381831002468No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC24691.813520977788224No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT19711.4477318133740746No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC18241.3397579033964038No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTACAGTACC15161.1135268539193794No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC14271.0481548948172523No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC12700.9328358208955223No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGAATACC11250.8263309437066635No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC10250.7528793042660712No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCCCGGATACC9820.7212950993066165No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTATCCAGCTACC9300.6831002467975085No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC9210.6764895992478552No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC8330.6118521565401339No Hit
TTACCGCGGCTGCTGGCACTGAGCCAGGATCAAACTCTTGTAGAACCATG8120.5964273122576096No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC7150.525179222000235No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAATTACC5860.430426607121871No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC5590.41059466447291104No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTCGATGGGTACC5490.40324950052885183No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTCAGTAATTACC5460.401045951345634No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC5310.3900282054295452No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCCACTACC4910.36064754965330825No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC4890.3591785168644964No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC4540.3334704430602891No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC4010.2945410741567752No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACAGTACC3790.27838171347984486No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC3550.2607533200141027No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTACTTACC3400.24973557409801386No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCATGAGTTACC3220.23651427899870725No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGATTACC3180.23357621342108356No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC3180.23357621342108356No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC2940.2159478199553414No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC2790.20493007403925256No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAATTACC2660.19538136091197555No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTATTCCGGTACC2630.19317781172875778No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC2560.18803619696791632No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGCCTTTCTAAGTAATTACC2500.18362909860148077No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTCCTTCTTCTGTACCTACC2270.16673522153014456No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCGTATGGTACC2220.16306263955811495No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2050.15057586085321426No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC1910.14029263133153133No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC1900.1395581149371254No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCAGGTACC1880.13808908214831356No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTTAGGTACC1770.1300094018098484No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC1730.1270713362322247No Hit
TTACCGCGGCTGCTGGCACAGAGTTTGCCGGTGCTTCCTTTCCTGGTACC1710.12560230344341286No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCATGGCTTTCTATTCCGGTACC1690.124133270654601No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGCTACC1680.1233987542601951No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTTCCAGTACA1650.12119520507697733No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAGTTACC1600.1175226231049477No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTTCTTGTTAGGTACC1560.114584557527324No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCCTCGAGTCCT1560.114584557527324No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCCTACGGTACC1550.11385004113291809No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC1500.11017745916088847No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTACTTTCTAAGTAATTACC1400.10283229521682925No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC1380.1013632624280174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)