FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110893
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG4504540.620237526264056No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1814416.36171805253713No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG105379.501952332428559No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG28412.561929066758046No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG19521.7602553813135184No Hit
AGAGTTTGATCCTGGCTCAGAAGGAACGCTAGCTATATGCTTAACACATG17601.587115507741697No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTACTTAATGCATG17471.5753924954686047No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG14641.3201915359851388No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG11181.008179055485919No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG8600.7755223503737837No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGACAGGCTTAACACATG7690.6934612644621392No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCTTAACACATG5190.46801872074883No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG5020.4526886277763249No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG4640.41842136113190187No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG3860.34808328749334944No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG3680.3318514243459912No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG3280.2957806173518617No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG2610.23536201563669484No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGGATGCTTTACACATG2480.22363900336360276No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG2260.20380005951683156No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG2120.1911752770688862No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2110.19027350689403297No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG2080.18756819636947328No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG2060.1857646560197668No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG2020.18215757532035387No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG1900.17133633322211503No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG1780.16051509112387616No Hit
AGAGTTTGATCCTGGCTCGGGATGAACGCTGGCGGCGTGCCTAATACATG1610.14518499815137115No Hit
AGAGTTTGATCCTGGCTCAGGACGGACGCTGGCGGCGTGCCTAATACATG1510.13616729640283876No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG1510.13616729640283876No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG1480.13346198587827907No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAATACATG1440.1298549051788661No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGTGGCGTGCCTAATACATG1420.12805136482915966No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGACATGGATAAGGCATG1350.12173897360518698No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAGTACATG1200.10821242098238842No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTCATACATG1120.10099825958356254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)