FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110893
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3405430.708881534452132No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1224111.038568710378472No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC51804.671169505739767No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC24672.224667021362935No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC23142.0866961846103904No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC15441.3923331499733977No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCAGGTACC14411.2994508219635144No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCCTCGAGTCCT13101.1813189290577404No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC11191.0090808256607722No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC10090.9098861064269161No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC9400.8476639643620427No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC8480.7647011082755448No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC8360.7538798661773061No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCACTACC7390.6664081592165421No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC7370.6646046188668356No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC7100.6402568241457982No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCCCTTTCTGGTATGGTACC6170.5563921978844472No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTCGCTCGGTACC6030.5437674154365019No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCTCCGGGTACT5850.5275355522891436No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC4930.44457269620264583No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCATGTGATACA4700.42383198218102136No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCGTATGGTACC4680.4220284418313148No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC4580.4130107400827825No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC3980.3589045295915883No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGGAGGTACC3770.3399673559196703No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCTCCTACC3610.32553903312201854No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCACTTCTTCTGTTGGTACC3530.3183248717231926No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCCAGGTACC3470.3129142506740732No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC3420.308405399799807No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC3410.30750362962495376No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTCAGGTACC3360.3029947787506876No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC3280.2957806173518617No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTACAGTACC3220.2903699963027423No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAATTGATTACC2880.2597098103577322No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC2750.24798679808464016No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC2130.19207704724373945No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC2050.18486288584491356No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTGCAGGTACC2030.18305934549520708No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC1940.17494341392152796No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1880.16953279287240855No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC1840.1659257121729956No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC1690.15239915955019703No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCCCTTTCTGGTAAGATACC1670.15059561920049055No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTCTCTGGTAAGATACC1640.14789030867593084No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATAAC1640.14789030867593084No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTCCTGGTAAGATACC1460.1316584455285726No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCCGGTAAGATACC1410.12714959465430642No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC1400.12624782447945318No Hit
CTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCAGGTACC1350.12173897360518698No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGCTACC1320.11903366308062727No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAGGATACC1320.11903366308062727No Hit
TTACCGCGGCTGCTGGCACGTAGCTAGCCGTCCCTTTCTGGTAAGATACC1300.1172301227309208No Hit
TTACCGCGGCTGCTGGCCCGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1270.1145248122063611No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC1240.11181950168180138No Hit
TACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACCG1210.10911419115724166No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGGTAGTTACC1200.10821242098238842No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTCGTAAGATACC1110.1000964894087093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)