FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169224
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG8660051.17477426369782No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG4550026.887439133928993No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG16010.9460832978773697No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG13780.8143052994847068No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG11470.6777998392662978No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG6720.3971067933626436No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATG4980.29428449865267337No Hit
AGAGTTTGATCCTGGCTCGGGATGAACGCTGGCGGCGTGCCTAATACATG4550.2688743913392899No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGTGTGCCTAATACATG4540.2682834586110717No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG4090.24169148584125183No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG3530.20859925306103153No Hit
AGAGTTTGATCCTGGCTCAGGATGAGCGCTGGCGGCGTGCCTAATACATG3530.20859925306103153No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACACG3490.20623552214815866No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACGTG3480.20564458941994043No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG3370.19914432940954002No Hit
AGAGTTTGATCCTGGCTCAGGGTGAACGCTGGCGGCGTGCCTAATACATG3030.17905261665012057No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCGTGCCTAATACATG2930.17314328936793835No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAGTACATG2910.17196142391150193No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2780.16427929844466507No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG2780.16427929844466507No Hit
AGAGTTTGATCCTGGCTCAGGATGGACGCTGGCGGCGTGCCTAATACATG2640.15600624024961No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG2580.15246064388030067No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGCGTGCCTAATACATG2440.1441875856852456No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGCGCCTAATACATG2410.14241478750059092No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2330.13768732567484518No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCCAATACATG2240.13236893112088122No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCATAACACATG2240.13236893112088122No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG2190.1294142674797901No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAATACATG2030.11995934382829859No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG1980.1170046801872075No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTCATACATG1880.11109535290502529No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG1840.10873162199215242No Hit
AGAGTTTGATCCTGGCCCAGGATGAACGCTGGCGGCGTGCCTAATACATG1820.10754975653571597No Hit
AGAGTCTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1780.1051860256228431No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG1730.10223136198175199No Hit
GAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATGC1730.10223136198175199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)