FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169224
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC6967641.17382877133267No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2414414.267479790100696No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC115236.809317827258544No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC44312.6184229187349315No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC20061.1854110528057487No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC15510.9165366614664586No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGATTAGGTACC10430.6163428355316031No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCATACGGTACA9410.5560676972533447No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC8570.5064293480830142No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTACCACTACC8160.48220110622606727No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC7200.42547156431711813No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC6990.41306197702453556No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTCGGTACC6870.4059707842859169No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC5910.3492412423769678No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGATTAGGTACC5090.3007847586630738No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC4950.2925117004680187No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGACTAGGTACC4890.2889661040987094No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC4690.277147449534345No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTCTGATTAGGTACC4190.24760081312343402No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCCTTCTGATTAGGTACC3560.2103720512456862No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTAAC3410.2015080603224129No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGCTTAGGTACC3230.19087127121448494No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC3210.1896894057580485No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGTTTAGGTACC3110.1837800784758663No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTGGCTTTCTGATTAGGTACC2980.17609795300902945No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGTGGCTTTCTGATTAGGTACC2820.16664302935753794No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTGATTAGGTACC2780.16427929844466507No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTCCTTATTCATATGGTACC2750.1625065002600104No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGGTTAGGTACC2750.1625065002600104No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTCTCTGATTAGGTACC2690.15896090389070108No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC2620.15482437479317354No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATCAGGTACC2620.15482437479317354No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGCCTTTCTGATTAGGTACC2600.1536425093367371No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCGGCTTTCTGATTAGGTACC2410.14241478750059092No Hit
TACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACCG2390.1412329220441545No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCCGATTAGGTACC2320.13709639294662695No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC2200.13000520020800832No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTTAGGTACC2090.1235049401976079No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC2070.12232307474117146No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGATACC2040.12055027655651682No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTGGGTACC2030.11995934382829859No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGAGTAGGTACC2010.11877747837186214No Hit
TTACCGCGGCTGCTGGCACGTAGCTAGCCGTGGCTTTCTGATTAGGTACC1930.11405001654611639No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC1930.11405001654611639No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATAAGGTACC1860.10991348744858885No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC1830.1081406892639342No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC1750.10341322743818843No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGACCTTATTCGTATAGTACT1750.10341322743818843No Hit
TTACCGCGGCTGCTGGCCCGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1710.10104949652531556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)