FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136631
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG5098637.31656798237589No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG2691619.69977530721432No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG93106.813973402814881No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCATAACACATG91076.665398042903879No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG65884.8217461630230325No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG19951.460137157746046No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG11240.8226537169456418No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCTTAACACATG9010.659440390540946No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG7790.5701487949294084No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCCTAACACATG5240.38351472213480103No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTGCGCCTAAAACATG4460.3264266528093917No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCTTAACACATG3830.28031705835425347No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG3450.25250492201623354No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCATAACACATG3190.2334755655744304No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG2880.2107867175092036No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG2740.20054014096361736No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2690.19688064934019364No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG2510.18370647949586844No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2490.18224268284649897No Hit
AGAGTTTGATCCTGGCTCAGACTGAACGCTGGCAGTATGGATAAGGCATG2300.168336614677489No Hit
AGAGTTTGATCCTGGCTCAGGATAAACGCTGGCGGCGTGCATAACACATG2110.15443054650847904No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG2070.1515029532097401No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG2060.15077105488505538No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG2010.1471115632616317No Hit
AGAGTTTGATCCTGGCTCAGGACGGACGCTGGCGGCGTGCCTAATACATG1980.1449158682875775No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG1930.1412563766641538No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG1760.12881410514451333No Hit
AGAGTTTGATCCTGGCTCGGGATGAACGCTGGCGGCGTGCCTAATACATG1740.12735030849514384No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAATACATG1730.12661841017045913No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG1580.1156399353001881No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATG1540.11271234200144915No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACGCATG1480.10832095205334075No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAGTACATG1430.10466146042991707No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCCAATACATG1420.10392956210523235No Hit
AGAGTTTGATCCTGGCTCAGGATAAACGCTGGCGGCGTGCGTAACACATG1400.10246576545586286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)