FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136631
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3804227.84287606765668No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1589611.634255769188544No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGACCTTATTCGTATAGTACT72395.298211972392795No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC53063.883452510777203No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC40882.992000351311196No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC30922.2630296199252No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC26481.9380667637651776No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC15321.1212682334170139No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC14001.0246576545586286No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC11700.8563210398811397No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC10370.75897856269807No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC9940.7275069347366264No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCCTCACCCGGTACC9150.6696869670865324No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC8480.6206497793326551No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC8270.6052799145142757No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTTTCTTATGGTACC7160.5240392004742701No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC6700.4903718775387723No Hit
CTACCGCGGCTGCTGGCACGTAATTAGCCGGACCTTATTCGTATAGTACT5770.422305333343092No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGAAGGTACC5300.3879061120829095No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC4490.3286223477834459No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTTTTCTGCAGGTACC4380.32057146621191385No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC4160.3044697030688497No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGATACC3960.289831736575155No Hit
TTACCGCGGCTGCTGGCACAGAATTAGCCGGTGCTTTCTCGTGAGGTACC3860.2825127533283076No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC3790.2773894650555145No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCCTTTGGAGGTACC3780.27665756673082975No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC3340.24445404044470143No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTTACGATACC3180.23274366724974566No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAATTACC3100.22688848065226777No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC3070.22469278567821357No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC2740.20054014096361736No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC2690.19688064934019364No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC2670.1954168526908242No Hit
TTACCGCGGCTGCTGGCACGTACTTAGCCGGACCTTATTCGTATAGTACT2430.17785129289839055No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC2380.1741918012749669No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGATTAGGTACC2140.15662624148253323No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGGCGCCCCTTTCTTGTATGGTACC2020.14784346158631642No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCTAGGTACC2010.1471115632616317No Hit
TNACCGCGGCTGCTGGCACGTAATTAGCCGGACCTTATTCGTATAGTACT1960.14345207163820803No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1850.13540119006667592No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC1740.12735030849514384No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGACCTTATTCGTATAGTACC1650.12076322357298123No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGTCCTTATTCGTATGGTACT1630.11929942692361177No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTCGGTACC1630.11929942692361177No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTCCTTCTTCTGTACCTACC1610.1178356302742423No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCCTTCTGGTAAGATACC1610.1178356302742423No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCCGGTAAGATACC1590.11637183362487283No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTCCTGGTAAGATACC1580.1156399353001881No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGTTAAGATACC1560.11417613865081863No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTGTGAAGGTACC1520.11124854535207969No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCCCTTTCTGGTAAGATACC1510.11051664702739497No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATAAC1510.11051664702739497No Hit
TACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACCG1500.10978474870271021No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC1430.10466146042991707No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC1390.10173386713117813No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGACCTTATTCGTATAGTACT1380.1010019688064934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)