FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137184
Sequences flagged as poor quality0
Sequence length300
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1821913.280703289013296No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1720412.540821087007231No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC119238.691246792628878No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC97077.075898063914159No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCAACCTCTTATTCGTTAGGTACC63764.647772334966177No Hit
TTACCGCGGCTGCTGGCACGGAGTTGGCCGGCGCTATTACCTGGGTACCG54323.959645439701423No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC47163.437718684394682No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC20891.5227723349661768No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC15031.0956088173547935No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC14831.081029857709354No Hit
TTACCGCGGCTGCTGGCACGAGTTTAGCAACCCCTTATTCATGTGGTACT12800.9330534173081408No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCGAGTAAC12460.9082691859108933No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC12450.9075402379286214No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC11080.8076743643573594No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC9880.7202006064847212No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC8440.6152320970375554No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGCTACC5350.389987170515512No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCAACCTCTTATTCGTTAGGTACC5250.3826976906927922No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCACCTCTTATTCGTTAGGTACC4960.3615581992069046No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC4830.3520818754373688No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC4720.34406344763237695No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC4600.33531607184511314No Hit
CTACCGCGGCTGCTGGCACGGAGTTGGCCGGCGCTATTACCTGGGTACCG4450.3243818521110334No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC3520.2565896897597387No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCACCCTCTTATTCGTTAGGTACC3060.22305808257522744No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGATTAGGTACC2850.20775017494751574No Hit
TTACCGCGGCTGCTGGCACGGAGTTGGCCGGCGCTCTTACCTGGGTACCG2750.2004606951247959No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC2560.18661068346162818No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCCTCTACC2290.16692908794028458No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC2260.16474224399346862No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTCGGTACC2020.14724749241894097No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC2020.14724749241894097No Hit
TNACCGCGGCTGCTGGCACGGAGTTGGCCGGCGCTATTACCTGGGTACCG1880.1370422206671332No Hit
TNACCGCGGCTGCTGGCACAGAGTTAGCAACCTCTTATTCGTTAGGTACC1870.1363132726848612No Hit
TTACCGCGGCTGCTGGCACGGAGTTGGCCGGCGCTATTCCCTGGGTACCG1710.12465010496850945No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC1670.1217343130394215No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGATTAGGTACC1660.12100536505714954No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCAACCGCTTATTCGTTAGGTACC1600.1166316771635176No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC1580.11517378119897365No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC1530.11152904128761372No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC1520.11080009330534174No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC1480.1078843013762538No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCCTCTCCATCTACC1430.10423956146489387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)