FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96202
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG3250433.787239350533255No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1355414.089104176628345No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG1059711.015363505956218No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG75027.798174674123199No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG47794.967672189767365No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG28002.910542400365897No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG18741.9479844493877467No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG9861.0249267167002765No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG5820.6049770275046257No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGTATGCCTTACACATG5620.5841874389305836No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG4840.5031080434918193No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG3800.3950021829068002No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG2690.2796199663208665No Hit
AGAGTTTGATCCTGGCTCAGGATAAACGCTGGCGGCGTGCATAACACATG2280.23700130974408018No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG2240.23284339202927173No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAACACATG2230.23180391260056965No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACACATG2120.22036963888484645No Hit
AGAGTTTGATCCTGGCTCAGGACAAACGCTGGCGGCATGCCTAACACATG2020.20997484459782542No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG1950.20269848859691067No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG1840.1912642148811875No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1560.16215879087752855No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG1480.15384295544791168No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG1460.1517639965905075No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG1420.14760607887569904No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG1370.1424086817321885No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG1220.12681649030165693No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG1190.1236980520155506No Hit
AGAGTTTGATCCTGGCTCAGGATAAACGCTGGCGGTGTACATAAGACATG1190.1236980520155506No Hit
AGAGTTTGATCCTGGCTCAGGACGGACGCTGGCGGCGTGCCTAATACATG1130.11746117544333798No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG1030.10706638115631692No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAACACATG1010.10498742229891271No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAATACATG980.10186898401280638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)