FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140907
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG8574360.8507739147097No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG2207115.663522749047242No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG39372.7940414599700514No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG11570.8211089583909955No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG6430.45632935198393265No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG6080.4314902737266424No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG5100.3619408546062296No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATG4530.3214886414443569No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG4250.3016173788385247No Hit
AGAGTTTGATCCTGGCTCAGGATGAGCGCTGGCGGCGTGCCTAATACATG3330.2363260874193617No Hit
AGAGTTTGATCCTGGCTCGGGATGAACGCTGGCGGCGTGCCTAATACATG3260.23135827176790366No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACACG3210.22780983201686217No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACGTG2860.2029707537595719No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGCGTGCCTAATACATG2850.20226106580936362No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCGTGCCTAATACATG2800.19871262605832218No Hit
AGAGTTTGATCCTGGCTCAGGGTGAACGCTGGCGGCGTGCCTAATACATG2620.18593824295457287No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGCGCCTAATACATG2480.17600261165165676No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG2460.17458323575124018No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2300.1632282285479075No Hit
AGAGTTTGATCCTGGCTCAGGATGGACGCTGGCGGCGTGCCTAATACATG2190.15542166109561625No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG2150.15258290929478308No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCCAATACATG2120.15045384544415819No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAATACATG2060.14619571774290846No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG2000.14193759004165868No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAGTACATG1950.1383891502906172No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1900.13484071053957575No Hit
AGAGTTTGATCCTGGCTCAGCCACTTCATGTGTGTGTCTTAGGCCAGTTA1880.13342133463915914No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATGCATG1760.12490507923665964No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTCATACATG1710.12135663948561817No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG1650.1170985117843684No Hit
AGAGTCTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1640.11638882383416013No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1610.11425975998353524No Hit
AGAGTTTGATCCCGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1580.11213069613291037No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTACTACATG1560.11071132023249376No Hit
AGAGTTTGATCCTGGCTCAGGTGATCCTCCCACATCAGCCTCTCGAGTAG1550.11000163228228547No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCAGCGTGCCTAATACATG1540.1092919443320772No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG1540.1092919443320772No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCGTGCCTAATACATG1530.1085822563818689No Hit
AGAGTTTGATCCTGGCTCAGGATGCACGCTGGCGGCGTGCCTAATACATG1530.1085822563818689No Hit
GAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATGC1510.1071628804814523No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG1430.10148537687978595No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG1420.10077568892957767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)