FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152212
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC4085126.83822563267022No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3049720.03587102199564No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC57903.8039050797571807No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC50033.2868630594171284No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC33352.191023046803143No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT27781.8250860641736526No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC26511.7416498042204294No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT24791.6286495151499225No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC20371.3382650513757128No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC18441.2114682153838068No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC12170.7995427430163192No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC10640.6990250440175544No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC8380.550547920006307No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC7370.4841930990986256No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGATTAGGTACC5410.35542532783223396No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTCGGTACC4080.2680471973300397No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCTCCTACC4050.2660762620555541No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC3840.252279715134155No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCGTTAGGTACC3830.2516227367093265No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC3790.24899482301001236No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC3310.21745985861824296No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGACTAGGTACC3300.21680288019341443No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGATTAGGTACC3240.21286100964444327No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC2980.19577957059890155No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC2870.1885528079257877No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2750.18066906682784536No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC2700.1773841747037027No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCCTCGAGTCCT2510.1649015846319607No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTATTCATCAGGTACC2470.16227367093264658No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC2470.16227367093264658No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCATGGCTTTCTGATTAGGTACC2440.160302735658161No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCCTTCTGATTAGGTACC2240.14716316716159042No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC2190.14387827503744777No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTCTGATTAGGTACC2190.14387827503744777No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT2180.14322129661261926No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC2130.1399364044884766No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGCTTAGGTACC2030.13336662024019133No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC2020.1327096418153628No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTAAC2010.13205266339053426No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTCTTCTCAGATACC1950.1281107928415631No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC1920.1261398575670775No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1870.12285496544293487No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC1860.12219798701810633No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC1740.11431424592016398No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTCTCTGATTAGGTACC1680.11037237537119282No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGTGGCTTTCTGATTAGGTACC1670.10971539694636429No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT1660.10905841852153575No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGGTTAGGTACC1650.10840144009670721No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC1640.1077444616718787No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCGGCTTTCTGATTAGGTACC1630.10708748324705017No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC1610.1057735263973931No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTGATTAGGTACC1610.1057735263973931No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC1610.1057735263973931No Hit
TTACCGCGGCTGCTGGCCCGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1570.10314561269807898No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGGGCTTCCTCACCCGGTACC1550.10183165584842194No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGTTTAGGTACC1530.10051769899876487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)