FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117218
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG4690640.01603849238172No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG2841324.239451278813835No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG42233.602689006807828No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG25312.1592246924533773No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCATAACACATG19581.6703919193297958No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG18601.586787012233616No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG12381.0561517855619444No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG11921.0169086659045539No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGTTGGCGGCGTGCCTAATACATG10640.9077104199013802No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG9800.8360490709617977No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAATACATG7360.627889914518248No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATG7040.6005903530174547No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG5630.48030166015458375No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG5530.4717705471855858No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGTGTGCTTAACACATG5490.46835810199798666No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG4190.3574536334010135No Hit
AGAGTTTGATCCTGGCTCAGAAGGAACGCTAGCTATATGCTTAACACATG3960.33783207357231826No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGTGTGCCTAATACATG3650.3113856233684246No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG3460.2951765087273286No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG2880.24569605350714055No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG2860.24398983091334098No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCCTAACACATG2770.2363118292412428No Hit
AGAGTTTGATCCTGGCTCGGGATGAACGCTGGCGGCGTGCCTAATACATG2240.19109693050555374No Hit
AGAGTTTGATCCTGGCTCAGGGTGAACGCTGGCGGTATGGATAAGGCATG2150.18341892883345562No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2040.1740347045675579No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG1930.16465048030166016No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG1920.16379736900476036No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG1800.15356003344196284No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG1780.15185381084816327No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG1770.15100069955126344No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGTGTGCCTAATACATG1740.14844136566056407No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG1680.14332269787916532No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG1630.13905714139466635No Hit
AGAGTTTGATCCTGGCTCAGGACGGACGCTGGCGGCGTGCCTAATACATG1440.12284802675357027No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATG1440.12284802675357027No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCCTAACACATG1370.11687624767527172No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCCAATACATG1350.11517002508147213No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAGTACATG1340.11431691378457234No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAATACATG1330.11346380248767253No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACGTG1310.11175757989387296No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACACG1310.11175757989387296No Hit
AGAGTTTGATCCTGGCTCAGGATGAGCGCTGGCGGCGTGCCTAATACATG1230.10493268951867461No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGCGTGCCTAATACATG1200.10237335562797523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)