FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117218
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2478921.14777593884898No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1498412.783019672746507No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC64855.532426760395161No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAATTACC62825.359245167124503No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC47694.068487774915115No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAAATACC18741.598730570390213No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC17381.4827074340118411No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGACCTTATTCGTATAGTACT16081.371802965414868No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC14601.2455424934736985No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC14531.2395707143954No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC10270.876145301916088No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC9950.8488457404152945No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC9180.7831561705540104No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTACTTTTGAGGTACC8250.7038168199423297No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC7790.6645737002849391No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCACGCTACC7150.6099745772833524No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC7010.5980310191267553No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTCCGGTACC6680.5698783463290621No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC5670.4837141053421829No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTGCGGTACC5330.4547083212475899No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC5060.43167431623129554No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTTTTCTGCAGGTACC4790.4086403112150011No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTACTGCTACC4480.38219386101110747No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAATTACC4420.3770751932297087No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC4340.3702503028545104No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC3420.2917640635397294No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC3390.28920472964903005No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTCAGTAATTACC3350.28579228446143085No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCCTCGAGTCCT3300.28152672797693185No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC2840.24228360831954135No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC2600.22180893719394632No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTAAATACC2550.21754338070944734No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC2380.20304048866215088No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC2370.20218737736525105No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC2330.1987749321776519No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC2160.18427204013035542No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTACTTACC2130.18171270623965605No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC2080.17744714975515705No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGGAGCCCCTTATTCATGAGGTAAC1990.16976914808305893No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC1810.15441314473886264No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAATTACC1790.15270692214506304No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC1760.15014758825436367No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGCCTTTCTAAGTAATTACC1690.14417580917606512No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGGTTAGGTACC1640.13991025269156615No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGCTACC1580.13479158491016738No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAGGATACC1510.1288198058318688No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1400.1194355815659711No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC1350.11517002508147213No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATAAC1330.11346380248767253No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC1270.10834513470627377No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTAAATACA1270.10834513470627377No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCCCTTTCTGGTAAGATACC1200.10237335562797523No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAAATACC1190.10152024433107544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)