FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80912
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG2108826.062883132291876No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1812522.400879968360687No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG891811.021850899742931No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG60737.505685188847143No Hit
AGAGTTTGATCCTGGCTCAGTGTGTGCCAGCAGCCGCGGTAAAGACCAAG29793.681777733834289No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGCGCCTAACACATG13291.6425252125766263No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAACACATG10091.2470338145145343No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG9841.2161360490409334No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG8961.107375914573858No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCATAACACATG8221.0159185287719992No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG4770.5895293652363062No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGCGCCTAACACATG3910.4832410520071188No Hit
AGAGTTTGATCCTGGCTCAGTGTGTGCCAGCAGCCGCGGTAGAGACCAAG3270.4041427723947004No Hit
AGAGTTTGATCCTGGCTCAGGATGAATGCTGGCGGCGTGGATAAGGCATG2980.3683013644453233No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG2960.3658295432074352No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG2170.26819260431085623No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG2080.2570694087403599No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG1980.2447103025509195No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGTCGGCATGCCTTACACATG1660.2051611627447103No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG1610.19898160964999012No Hit
AGAGTTTGATCCTGGCTCGGGATGAACGCTGGCGGCGTGCCTAATACATG1360.16808384417638916No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTTGCGGCGTGCCTAAGAAATG1290.1594324698437809No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG1290.1594324698437809No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATG1110.13718607870278823No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG1100.13595016808384416No Hit
AGAGTTTGATCCTGGCTCAGGATGGACGCTGGCGGCGTGCCTAATACATG880.10876013446707535No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG880.10876013446707535No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG870.10752422384813129No Hit
AGAGTTTGATCCTGGCTCAGGGTGAACGCTGGCGGCGTGCCTAATACATG830.10258058137235515No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG820.10134467075341111No Hit
AGAGTTTGATCCTGGCTCAGTGTGTGCCAGCAGCCGCGGTCAAGACCAAG820.10134467075341111No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)