FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170409
Sequences flagged as poor quality0
Sequence length300
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC3101118.197982500924248No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC160699.429666273494945No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC123917.271329565926682No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC92665.437506234999325No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC84654.967460638816025No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT32721.9200863804141801No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC30231.7739673374058882No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTAAGATACC23331.3690591459371277No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC19431.1401979942373934No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC18401.0797551772500278No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC15400.9037081374810015No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC13840.812163676801108No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACA12790.7505472128819487No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTAGTCAGGTACC12100.7100563937350727No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCCTCCTCAAGTACC9360.549266764079362No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC8750.51347053265966No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC8020.4706324196491969No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCAGGTAAC7530.44187806982025596No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC7280.4272074831728371No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC7100.41664466078669554No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTCCTCAGGTACC6890.4043213680028637No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC6700.3931717221508254No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC5990.3515072560721558No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC5150.30221408493682844No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGATTAGGTACC4860.2851962044258226No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC4770.27991479323275176No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCCCTTTCTGGTATGGTACC4570.2681783239148167No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGGATACC4420.2593759719263654No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC4370.2564418545968816No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCATAAGGTACC4350.2552682076650881No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCAGCTAAT4280.25116044340381083No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTACAGTACC3740.21947197624538609No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC3740.21947197624538609No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGGTAGTTACC3610.2118432711887283No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC3560.20890915385924452No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTCGGTACC3530.20714868346155424No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTAGATACC3180.18660986215516787No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC3170.18602303868927111No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGGGGCTTCTTCTGCGGGTACC3070.18015480403030357No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC3040.1783943336326133No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC2890.16959198164416198No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCCCGTACC2890.16959198164416198No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTAAAGTACC2870.16841833471236847No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTTCACGTACC2870.16841833471236847No Hit
TTACCGCGGCTGCTGGCACAGAGTTTGCCGGTGCTTCCTCTCCTGGTACC2770.16255010005340093No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC2710.1590291592580204No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGATTAGGTACC2710.1590291592580204No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2690.1578555123262269No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGTTACC2680.15726868886033013No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGGTGCTTCTTCTGTAGGTACC2660.15609504192853663No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC2550.14963998380367233No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGACTAGGTACC2540.14905316033777558No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGTTACC2470.1449453960764983No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT2280.13379575022445997No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCGAGTACC2220.13027480942907943No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACC2190.1285143390313892No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2140.12558022170190541No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTATTACTCAGGTACCG2050.12029881050883463No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC2040.11971198704293787No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTAAC1950.11443057584986707No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGCTTAGGTACC1920.11267010545217683No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTCTGATTAGGTACC1870.10973598812269304No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC1820.10680187079320927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)