FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155701
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG2599116.692892145843636No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG117817.566425392258239No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG103786.665339336291995No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG80985.200994213267737No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG65434.202285149099878No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG62534.016030725557318No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG60523.8869371423433376No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG57683.704536258598211No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG47893.0757670149838474No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG38212.454062594331443No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAATACATG27001.7340929088445163No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG25981.6685827322881677No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCCTAATACATG25301.624909281250602No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAACACATG22751.461133839859731No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCCTAACACATG22101.4193871587208817No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG17581.1290871606476516No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATG15500.9954977810033332No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCTTAACACATG14500.9312721177127957No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG14130.9075086222952968No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAATACATG13560.8708999942196902No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG12400.7963982248026666No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG11080.711620349259157No Hit
AGAGTTTGATCCTGGCTCAGTGTGCCAGCAGCCGCGGTAAAGACCAAGTC10180.6538172522976732No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAACACATG9330.5992254385007162No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG9150.5876648191084194No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG8870.5696816333870689No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG8670.5568365007289613No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCTTAACACATG6740.43288097057822367No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG6550.4206780945530215No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGTATGCTTTACACATG6010.3859962363761312No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGTATGCTTAACACATG5990.3847117231103204No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCAGGCCTAACACATG5620.3609482276928215No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG4320.2774548654151226No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCAGGCCTAAAACATG4180.2684632725544473No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGCGTGCTTAACACATG4030.2588294230608667No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG3910.25112234346600215No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGTATGCCTTACACATG3900.25048008683309675No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTAGCGATAAGCTTAACACATG3750.24084623733951613No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGTATGCCTAACACATG3630.23313915774465158No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCTTAATACATG3320.21322920212458493No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGAATGCTTTACACATG3170.20359535263100428No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTAGCGTTAAGCCTAACACATG2850.18304314037803227No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG2660.17084026435283012No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG2540.1631331847579656No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCTTAACACATG2250.14450774240370967No Hit
AGAGTTTGATCCTGGCTCAGCCTGAACGCTGGCGGCGTGGCTAAGACATG1970.12652455668235915No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGACTAATACATG1880.12074424698621075No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACGTG1820.11689070718877849No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAACACATG1680.10789911432810323No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGTTAGCGGCGCGCTTAACACATG1650.1059723444293871No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGAGTGCCTAATACATG1610.10340331789776558No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCTTAACACATG1560.10019203473323872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)