FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155701
Sequences flagged as poor quality0
Sequence length300
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC74094.758479393195933No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC69424.458545545629122No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC60663.895928735204013No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC56783.646733161636727No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC55793.5831497549790945No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT39822.5574659122292087No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC37502.408462373395161No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC36712.3577240993956363No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC29661.9049331731973465No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTACAGTACC22431.4405816276067591No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC21911.4071842826956795No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTTTCTGCAGGTACC19441.2485468943680516No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCACATACC19301.2395553015073764No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCCTCTGGTACC18521.189459284140757No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGATACC17611.1310139305463678No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTTTTCTGCAGGTACC16521.0610079575596816No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAAGTACC14400.9248495513837419No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTCCGGTACC13560.8708999942196902No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGACTACC12350.7931869416381397No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGTAGGTACC12270.7880488885748967No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGTACCTACC11630.7469444640689527No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC11260.7231809686514538No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT10260.6589553053609161No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC10010.6428988895382818No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCTCCTACC9870.6339072966776064No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTGCGGGTACC9490.6095015446272022No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTCCTCAGGTACC9260.5947296420703785No Hit
TTACCGCGGCTGCTGGCACACTGAGCCAGGATCAAACTCTTGTAGAACCA9160.5883070757413247No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCGTATGGTACC8270.5311462354127462No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCTTCAGGTACA7760.498391147134572No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCGCCTACC7030.45150641293247956No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTCTTCTCCCGGTACC6650.42710066088207527No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC6600.4238893777175484No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTGCGGTACC6230.4001258823000495No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTGCGGGTACC6100.3917765460722796No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATAAGATACC5860.3763623868825505No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAAATACC5790.3718665904522129No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCGCTTCTTCTGCAGGTACC5590.3590214577941053No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC5460.3506721215663355No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCCTACAGTACC5340.34296504197147093No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTTACGATACC5290.3397537588069441No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATCAGGTACC5270.33846924554113333No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC5120.3288353960475527No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGTAGGTACC4760.30571415726295914No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGGGCTTCTTCTGCAGGTACC4730.303787387364243No Hit
TTACCGCGGCTGCTGGCACATAGTTAGCCGGTGCTTATTCCTCAGGTACC4700.30186061746552684No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC4670.29993384756681074No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGACCCTTATTCATGAGTTACC4650.298649334301No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC4610.2960803077693785No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTGCGGGTACC4500.28901548480741934No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTCTCGTTAGGTACC4470.2870887149087032No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC4430.28451968837708175No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC4380.28130840521255485No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCCTCAGGTACC4360.28002389194674404No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGACTTCTTCTGCTGGTACC4230.2716745557189742No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCATATAGTACC4140.2658942460228258No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC4090.26268296285829895No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCAACCGGTACC4040.259471679693772No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTCAGGTACC4030.2588294230608667No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTTACGGTACC3840.24662654703566453No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCATACGGTACA3810.24469977713694838No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC3720.23891946744079998No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTTTTCTGAAGGTACA3630.23313915774465158No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTAAGGTACC3580.22992787458012473No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGATGGTACC3550.22800110468140858No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC3480.22350530825107096No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTCCGGTACA3370.21644048528911183No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCGCATACC3280.21066017559296346No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCCCAGATACC3230.20744889242843653No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCATAAGGTACC3220.20680663579553116No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCGGGTACC3120.20038406946647744No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC3110.19974181283357204No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTTTCCGACTACA3110.19974181283357204No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC3040.19524601640323438No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT2970.19075021997289676No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCCTTAAGTACC2890.18561216690965376No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC2860.18368539701093764No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTACTTCTTCTGCAGGTACC2780.17854734394769461No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC2760.17726283068188387No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCGCAGAGTACC2690.17276703425154624No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGGGGCTTCCTCCTAGAGTACT2680.17212477761864084No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGACTACA2650.17019800771992472No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTCCTGTGGTACC2640.16955575108701934No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTCCGGTACC2590.16634446792249247No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC2550.16377544139087097No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCGCAAGGTACC2530.16249092812506022No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2520.16184867149215484No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCACTTCTTCTGTTGGTACC2440.15671061842891182No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGAAGGTACC2250.14450774240370967No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCGCTTCTTCTGTTGGTACC2200.1412964592391828No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCATACAGTACC2190.14065420260627742No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC2120.13615840617593977No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTTGGGGTACA2110.1355161495430344No Hit
TTACCGCGGCTGCTGGCACAGAATTGGCTGGGACTACTCCTACGGGTTTT2100.13487389291012902No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAAGTACC2100.13487389291012902No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC2060.13230486637850752No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTCCTCACCCGGTACC1990.12780906994816987No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC1970.12652455668235915No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC1920.12331327351783226No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTTCCAGTACA1760.11303716739134624No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC1750.11239491075844085No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGAGTAAC1660.10661460106229247No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCGAGTAAC1660.10661460106229247No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTTAGTGGGTACC1570.10083429136614408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)