FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191920
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2883615.025010421008753No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC155878.121613172155065No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC132616.9096498541058775No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC111865.8284701959149645No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT111395.803980825343894No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCGTATGGTACC91424.763443101292205No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC68853.5874322634431013No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC43272.2545852438516047No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC40452.107649020425177No Hit
TTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC18310.9540433513964152No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC15890.8279491454772822No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC15070.7852230095873279No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCACTACC12950.6747603167986661No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT12100.6304710295956649No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC11100.578365985827428No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC9070.47259274697790743No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC8290.43195081283868275No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC8200.4272613588995415No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC8130.42361400583576486No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC7300.38036681950812834No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTGCGGTACC7200.3751563151313047No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC6800.35431429762401No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC6640.3459774906210921No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT6420.33451438099208003No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC5870.3058566069195498No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT5450.2839724885368904No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC5340.27824093372238434No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC5120.2667778240933722No Hit
CTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCGTATGGTACC4970.2589620675281367No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC4630.24124635264693622No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC4120.21467278032513548No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC3880.20216756982075865No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCAGGTACT3530.1839308045018758No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGATCCTTATTCATAAGTTACC3520.18340975406419344No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGACCTTATTCGTATAGTACT3470.18080450187578156No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC3440.17924135056273446No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACC3410.17767819924968736No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC3250.16934139224676947No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTAAGGTACC3220.1677782409337224No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC3220.1677782409337224No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT3120.1625677365568987No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCACGCTACC3100.16152563568153397No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGTCGGTACC3040.15839933305543977No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTGCTCCTAAGGTACC2970.15475197999166318No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC2970.15475197999166318No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC2700.14068361817423927No Hit
TTACCGCGGCTGCTGGCACGGACTTAGCCGGTCCTTATTCGTATGGTACC2680.13964151729887453No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGGGCTTCTTAGTCAGGTACC2520.13130471029595667No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC2500.13026260942059192No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTTTCTTATGGTACC2480.12922050854522718No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC2450.12765735723218008No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC2370.12348895373072113No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTATCCAGCTACC2230.11619424760316797No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTATTCCGGTACC2200.11463109629012089No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTATTCTCCCGGTACA2190.1141100458524385No Hit
TNACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCGTATGGTACC2170.11306794497707379No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCGTAGGGTACA2160.11254689453939141No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCCCTTTCTGGTATGGTACC2130.11098374322634431No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTTTCTGCAGGTACC2080.10837849103793246No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACC2070.1078574406002501No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC2020.10525218841183827No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCATCTGGTACC1990.10368903709879115No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC1980.1031679866611088No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC1930.10056273447269697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)