FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222286
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG7189232.3421178121879No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG3077513.8447765491304No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG2398210.788803613362964No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG192098.64156986944747No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG86553.8936325274646175No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG40791.8350233482990381No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAACACATG28291.2726847394797691No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG25471.1458211493301422No Hit
AGAGTTTGATCCTGGCTCAGTGTGTGCCAGCAGCCGCGGTAAAGACCAAG23551.0594459390155024No Hit
AGAGTTTGATCCTGGCTCAGAAGGAACGCTAGCTATATGCTTAACACATG21150.9514769261222029No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG20340.9150373842707143No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG17860.8034694042809715No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG10760.48406107447162666No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG9430.42422824649325647No Hit
AGAGTTTGATCCTGGCTCAGGATCAACGCTGGCGGCGTGCCTAATGCATG7270.32705613488928675No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG7120.32030807158345553No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAACACATG6650.29916413989185103No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG6390.2874674968284102No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAACACATG5260.23663208659114834No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG5110.22988402328531712No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG5070.22808453973709544No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCTTAACACATG5030.22628505618887382No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGAATGCTTTACACATG4790.21548815489954384No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATG4670.21008970425487883No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG4040.18174783837038772No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG3860.17365016240339023No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG3680.16555248643639275No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG3360.15115661805061947No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG3270.14710778006712075No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG2750.12371449394023915No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCTTAACACATG2650.11921578506968501No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG2640.11876591418262959No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCTTAATACATG2580.1160666888602971No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG2570.11561681797324168No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATG2570.11561681797324168No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTTACACATG2540.11426720531207543No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG2400.10796901289329962No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGCGCCTAACACATG2380.10706927111918879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)