FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222286
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGATACC3624316.304670559549407No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCCCTTTCTGGTATGGTACC161977.286558757636559No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC152856.876276508642019No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGGTAGTTACC127765.747550453019983No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC73333.2989032147773587No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC59892.694276742574881No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC48952.2021179921362566No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC40991.8440207660401466No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC36061.6222344187218267No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC35801.6105377756583863No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT31181.4026974258387843No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC29711.3365664054416382No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC26291.1827105620686862No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC24651.1089317365915983No Hit
TTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC23661.064394518773112No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC20530.9235849311247672No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCCTCGAGTCCT17800.8007701789586388No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGATACC17650.7940221156528076No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC13150.5915802164778708No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC10060.45257011237774764No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGATACC10010.4503207579424705No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGTAGGTACC9950.44762153262013804No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTTTTCTGCAGGTACC9530.42872695536381056No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTATGGTACC9480.42647760092853354No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC8440.37969102867477034No Hit
CTACCGCGGCTGCTGGCACGTATTTAGCCGTCCCTTTCTGGTATGGTACC7890.3549481298867225No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC7850.35314864633850085No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGCTGCTTATTCCTCAGATACC7080.3185085880352339No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC6650.29916413989185103No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGCCTTCCTCCGTGGGTACC6070.273071628442637No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGGTAGTTACC5620.2528274385251433No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGTTACC5510.2478788587675337No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTTTTCTGAAGGTACA5240.2357323448170375No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCAGGTACC4700.2114393169160451No Hit
TNACCGCGGCTGCTGGCACGTATTTAGCCGTCCCTTTCTGGTATGGTACC4460.20064241562671511No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTACAGTACC4420.19884293207849346No Hit
TNACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC4230.1902953852244406No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTGCGGTACC4050.1821977092574431No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCTCCTACC3990.17949848393511064No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC3860.17365016240339023No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCATACTGTACC3860.17365016240339023No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTCGATACC3690.16600235732344817No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC3670.16510261554933736No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT3660.16465274466228194No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC3630.16330313200111568No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCCCAGATACC3490.15700493958233985No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGACTACC3390.1525062307117857No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCGCAAGGTACC3380.15205635982473029No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAAGTACC3290.1480075218412316No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTTAGTAGGTACC3260.14665790918006533No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGGTAGTTACC3260.14665790918006533No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGCTACC3230.14530829651889907No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTCCTTCTTCTGCAGGTACC3200.14395868385773283No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3150.14170932942245576No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATCAGGTACC2790.1255139774884608No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTTAGATACC2770.12461423571434999No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGGTACC2630.11831604329557417No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCGCATACC2630.11831604329557417No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC2590.11651655974735252No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCATCTGGTACC2560.11516694708618627No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTTCCTTTCTGGTAAGATACC2540.11426720531207543No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGCTAGATACC2500.11246772176385378No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGTACCTACC2400.10796901289329962No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCATACAGTACC2380.10706927111918879No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC2370.10661940023213339No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC2230.10032120781335756No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)