FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173682
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG6965140.1026013058348No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG2306613.280593268156746No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1947611.213597263965177No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG51012.9369767736437855No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG40522.3329993896892023No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG33781.944933844612568No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG30711.7681740191844864No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG20111.157863221289483No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG14420.8302529910986746No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG11620.6690388180698057No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG8450.4865213436049792No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGTGGCGTGCCTAATACATG8190.4715514561094414No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG7480.4306721479485497No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCCTAACACATG7030.40476272728319573No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG6920.3984293133427759No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG6750.3886413099803089No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG6410.3690653032553748No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATG6170.355246945567186No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG6060.3489135316267662No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG5950.34258011768634633No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG5050.2907612763556385No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGCGCCTAACACATG4630.26657915040130814No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG4450.2562153821351666No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG3900.22454831243306733No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG3690.21245724945590216No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG3590.20669960041915683No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG3460.19921465667138796No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCATAACACATG3280.1888508884052464No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG2750.1583353485104962No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG2460.14163816630393478No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTTACACATG2420.13933510668923665No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG2420.13933510668923665No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG2320.13357745765249135No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAATACATG2270.13069863313411867No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG2210.1272440437120715No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCCTAACACATG2200.12666827880839696No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG2120.1220621595790007No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAGTACATG2100.12091062977165164No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAACACATG2060.1186075701569535No Hit
AGAGTTTGATCCTGGCTCAGGACGGACGCTGGCGGCGTGCCTAATACATG2010.11572874563858086No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCCAATACATG1800.1036376826614157No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG1800.1036376826614157No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTACTACATG1740.1001830932393685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)