FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172375
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG7743644.92298767222625No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG2205012.791878172588833No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG147528.558085569253082No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCTTAACACATG93985.4520667150108775No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG59763.4668600435097896No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG53613.1100797679477883No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG18301.0616388687454676No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG14310.8301667875271936No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG12020.6973168963016678No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG9310.5401015228426396No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG5310.3080493110949964No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG5170.2999274836838288No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG4240.24597534445250183No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCCTAATACATG4010.23263234227701232No Hit
AGAGTTTGATCCTGGCTCAGAACGAGCGCTGGCGGCATGCCTAATACATG3170.18390137781000723No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACACATG3040.17635968092820886No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCATGCCTAATACATG2960.171718636693256No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACGTG2950.17113850616388687No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCTTTACACATG2880.16707759245830311No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACACG2820.16359680928208847No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG2720.1577955039883974No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG2500.145032632342277No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCCGGCGGCATGCCTAATACATG2480.1438723712835388No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG2360.13691080493110952No Hit
AGAGTTTGATCCTGGCTCGGAACGAACGCTGGCGGCATGCCTAATACATG2100.1218274111675127No Hit
AGAGTTTGATCCTGGCTCAGAACGGACGCTGGCGGCATGCCTAATACATG2070.12008701957940536No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCACGCCTAATACATG2060.11950688905003626No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCCAATACATG2000.1160261058738216No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAGTACATG1920.11138506163886874No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTCGCGGCAGGCTTAACACATG1740.1009427121102248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)