FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172375
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT6096035.36475707034082No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC121127.026540971718637No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC91685.318636693255983No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC81964.754749818709209No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC65713.8120377084844086No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC50622.936620739666425No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC41252.393038433647571No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT37912.1992748368382884No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCCGATACT17000.9862218999274838No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT16920.9815808556925308No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT16070.9322697606961565No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACC13640.7912980420594634No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC8870.5145757795503988No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC8340.48382886149383614No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC7850.45540246555474984No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC6090.3532994923857868No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT5370.31153009427121103No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC4930.28600435097897026No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC4880.28310369833212473No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGATACT4710.2732414793328499No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC4300.24945612762871647No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCCGATACT4130.23959390862944163No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC3680.21348803480783174No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATAAT3490.20246555474981873No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC3140.1821609862218999No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC3020.17519941986947063No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGCTCCTTATTCTCACAGTACC2910.16881798404641046No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCACGATACT2890.16765772298767223No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATCCTCCCGATACT2640.15315445975344452No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGCTACT2540.14735315445975344No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTACTCTCCCGATACT2530.14677302393038436No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCCCCCGATACT2530.14677302393038436No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC2440.14155184916606237No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2410.13981145757795505No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTATTCTCCCGATACT2360.13691080493110952No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC2360.13691080493110952No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTTCTTGTTAGGTACC2310.13401015228426397No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGAGCTTATTCTCCCGATACT2270.13168963016678753No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC2110.1224075416968818No Hit
TACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACTG2080.12066715010877448No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCCTATTCTCCCGATACT2050.11892675852066714No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1970.1142857142857143No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCCCAGTACC1950.11312545322697608No Hit
TTACCGCGGCTGCTGGCACGGGGTTAGCCGGAGCTTATTCTCCCGATACT1850.10732414793328499No Hit
TTACCGCGGCTGCTGGCATGGAGTTAGCCGGAGCTTATTCTCCCGATACT1840.10674401740391587No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATCCT1840.10674401740391587No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC1820.10558375634517765No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCGCCCGATACT1760.10210297316896302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)