FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152145
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG5518636.271977389989814No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG2011613.221597817871109No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG141749.31611291859739No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG140079.206349206349207No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG41292.7138584902560057No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG31632.078937855335371No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG16131.0601728614150974No Hit
AGAGTTTGATCCTGGCTCAGTGTGTGCCAGCAGCCGCGGTAAAGACCAAG14660.9635545039271748No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAACACATG14090.9260902428604292No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG11910.7828058759735779No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG10310.6776430378914851No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGCGCCTAACACATG4810.31614578198429133No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG4340.28525419829767656No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG4160.27342337901344116No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG4060.2668507016333103No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAACACATG3780.2484472049689441No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG3630.2385881888987479No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAATACATG3570.23464458247066944No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG3550.2333300469946433No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG3090.2030957310460416No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG3070.20178119557001545No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG2850.1873213053337277No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGAATGCTTTACACATG2710.17811955700154458No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGTGTGCCTAAAACATG2690.17680502152551844No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG2380.15642972164711294No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCTGCGTGCTTAACACATG2370.15577245390909988No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGTGTGCTTCACACATG2300.15117157974300832No Hit
AGAGTTTGATCCTGGCTCAGGACGGACGCTGGCGGCGTGCCTAATACATG2270.14919977652896907No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG2170.14262709914883828No Hit
AGAGTTTGATCCTGGCTCAGGATAAACGCTGGCGGCGTGCATAACACATG2000.1314535476026159No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG1850.12159453153241973No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG1750.11502185415228894No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG1650.10844917677215814No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAGTACATG1610.10582010582010583No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCCTAACACATG1550.10187649939202735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)