FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168313
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG8080948.01114590079198No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCTTAACACATG132207.854414097544455No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG127627.582302020640117No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG106556.330467640645701No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG74304.414394610042005No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG27451.6308900679091929No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG18851.1199372597482071No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG11030.6553266830250782No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG10090.5994783528307379No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG7530.4473807727270027No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG7210.42836857521403576No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG6420.3814322126038987No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCCTAATACATG3680.21864027139911948No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCATGCCTAATACATG3580.21269895967631733No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCTTTACACATG3060.1818041387177461No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG3000.17823935168406482No Hit
AGAGTTTGATCCTGGCTCAGAACGAGCGCTGGCGGCATGCCTAATACATG2930.17408043347810329No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG2890.17170390878898245No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG2850.16932738409986156No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACGTG2660.15803889182653746No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACACG2580.15328584244829574No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTCGCGGCAGGCTTAACACATG2570.15269171127601552No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGTAGGCCTAACACATG2280.13546190727988927No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAACACATG2230.13249125141848817No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCACGCCTAATACATG2190.13011472672936733No Hit
AGAGTTTGATCCTGGCTCGGAACGAACGCTGGCGGCATGCCTAATACATG2010.11942036562832342No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG1970.11704384093920255No Hit
AGAGTTTGATCCTGGCTCAGAACGGACGCTGGCGGCATGCCTAATACATG1960.11644970976692234No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCCGGCGGCATGCCTAATACATG1930.1146673162500817No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAGTACATG1900.11288492273324104No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCAGGCTTAACACATG1900.11288492273324104No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAATACATG1890.11229079156096083No Hit
AGAGTTCGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG1870.1111025292164004No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCCAATACATG1850.10991426687183997No Hit
GAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATGC1800.1069436110104389No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCTTAATACATG1710.10159643045991693No Hit
AGAGTTTGATCCCGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG1690.10040816811535652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)