FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168313
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT6353737.74931229316809No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC116356.912716189480314No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC110526.566337716040947No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC89895.3406451076268615No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC55073.27188036574715No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT33641.9986572635506468No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT18531.1009250622352402No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT17031.011805386393208No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCCGATACT16470.9785340407455159No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACC13830.8216834112635388No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC13330.791976852649528No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT10200.6060137957258204No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC6460.38380873729301956No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC6380.3790556879147778No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGATACT5110.3036010290351904No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC5080.30181863551834975No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGCTCCTTATTCTCACAGTACC4560.27092381455977854No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC4520.2685472898706577No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCCGATACT4470.2655766340092566No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC4190.24894096118541054No Hit
TNACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC3680.21864027139911948No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATAAT3500.2079459102980756No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC3480.20675764795351517No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC3200.19012197512966914No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCACGATACT3130.18596305692370763No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCCCAGTACC3030.18002174520090544No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC2870.170515646444422No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTACTCTCCCGATACT2780.16516846589390005No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC2770.16457433472161984No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGCTACT2770.16457433472161984No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATCCTCCCGATACT2440.14496800603637272No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCCCCCGATACT2360.14021495665813097No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCCTATTCTCCCGATACT2330.13843256314129032No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTATTCTCCCGATACT2310.1372443007967299No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGAGCTTATTCTCCCGATACT2190.13011472672936733No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTTTGGGTACC2150.12773820204024644No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC2130.126549939695686No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTCCCGATACT2060.12239102148972451No Hit
TACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACTG1950.11585557859464214No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATCCT1950.11585557859464214No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACGGTACC1830.10872600452727953No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTACC1830.10872600452727953No Hit
TTACCGCGGCTGCTGGCACGGGGTTAGCCGGAGCTTATTCTCCCGATACT1710.10159643045991693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)