FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244113
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG10284142.128440517301414No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG2946112.06859118522979No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG2865211.737187286215809No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG88733.6347920839938883No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG69932.864656941662263No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCTTAACACATG51202.0973893237967665No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG51122.094112152978334No Hit
AGAGTTTGATCCTGGCTCAGTGTGCCAGCAGCCGCGGTAAAGACCAAGTC39181.604994408327291No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG19970.8180637655511996No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG19850.8131480093235509No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG18750.7680869105701048No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG14510.5943968571931851No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG11210.4592135609328467No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG9810.40186307161027884No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCTTTACACATG6800.278559519566758No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATG4810.1970398954582509No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCATGCCTAATACATG4650.190485553821386No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCCTAATACATG4470.18311191947991298No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAATACATG4300.17614793149074404No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTAACACATG3760.15402702846632502No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCCTAACACATG3690.15115950400019662No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG3440.14091834519259522No Hit
AGAGTTTGATCCTGGCTCAGAACGAGCGCTGGCGGCATGCCTAATACATG3210.13149647908960196No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACGTG3140.12862895462347354No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACACG3060.1253517838050411No Hit
AGAGTTTGATCCTGGCTCGGAACGAACGCTGGCGGCATGCCTAATACATG2940.12043602757739244No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCTTAATACATG2740.11224310053131131No Hit
AGAGTTTGATCCTGGCTCAGAACGGACGCTGGCGGCATGCCTAATACATG2720.1114238078267032No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG2710.11101416147439915No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAACACATG2550.10445981983753425No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCACGCCTAATACATG2480.10159229537140588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)