FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244113
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT8678135.54952009929828No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC2592610.620491329834953No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC221259.063425544727236No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC85923.5196814589964487No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC54512.232982266409409No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC43861.7967089012055892No Hit
TTACCGCGGCTGCTGGCACACTGAGCCAGGATCAAACTCTTGTAGAACCA34731.4227017815519862No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT27881.142094030223708No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT25591.048285015546079No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT23890.9786451356543895No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCCGATACT22810.9344033296055515No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACC19400.7947139234698685No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC15130.6197949310360367No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT14240.5833364056809757No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCGAGTAAC7820.32034344750177174No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGATACT7080.2900296174312716No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC6930.28388492214671074No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC6550.2683183607591566No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCCGATACT5490.2248958474149267No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGAGCTTATTCTCCCGATACT5360.21957044483497398No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATAAT5040.20646176156124416No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC4990.2044135297997239No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC4540.18597944394604138No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC4170.17082252891079133No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCACGATACT4120.16877429714927103No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3810.1560752602278453No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTCCGGTACA3640.14911127223867635No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTTACGGTACC3590.14706304047715607No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATCCTCCCGATACT3490.14296657695411552No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC3450.14132799154489928No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC3400.139279759783379No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC3390.13887011343107497No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGCTACT3300.13518329626033845No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC3250.13313506449881815No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTACTCTCCCGATACT3240.13272541814651412No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTATTCTCCCGATACT3040.124532491100433No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCCCCCGATACT2980.12207461298660865No Hit
TTACCGCGGCTGCTGGCATGGAGTTAGCCGGAGCTTATTCTCCCGATACT2910.11920708852048026No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGTCCCTTTCTGGTAAGATACC2790.1142913322928316No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCCTATTCTCCCGATACT2630.1077369906559667No Hit
TACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACTG2600.10650805159905453No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTACTCTTACGGTACC2590.10609840524675047No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTTACGGTACC2560.1048694661898383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)