FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176521
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG6614937.473728338271364No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG2494914.133729131378136No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG112906.3958395884908885No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCTTAACACATG106566.036675523025589No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG94475.351771177367No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG70944.018785300332539No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG54033.0608256241467022No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG21681.2281824825374885No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG15620.8848805524555152No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG9460.5359135740223543No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG9140.5177854192985537No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG8940.5064553225961783No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG6710.3801247443646931No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCCTAATACATG3330.18864611009454965No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCATGCCTAATACATG3260.18468057624871828No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTAACACATG3230.18298106174336198No Hit
AGAGTTTGATCCTGGCTCAGAACGAGCGCTGGCGGCATGCCTAATACATG3040.1722174698761054No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG2860.16202038284396758No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG2730.1546558199874236No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACGTG2360.13369514108802918No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG2330.1319956265826729No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACACG2320.1314291217475541No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAATACATG2270.1285965975719603No Hit
AGAGTTTGATCCTGGCTCGGAACGAACGCTGGCGGCATGCCTAATACATG2210.12519756856124767No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCTTTACACATG2200.12463106372612891No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTCGCGGCAGGCTTAACACATG2120.12009902504517876No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAGTACATG1950.11046844284815971No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCAGGCTTAACACATG1910.10820242350768465No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG1900.10763591867256586No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCCGGCGGCATGCCTAATACATG1880.10650290900232834No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCCAATACATG1840.10423688966185325No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCACGCCTAATACATG1790.10140436548625943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)