FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176521
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT5072628.736524266234614No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC2121812.020099591550014No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC98355.57157505339308No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC86504.900266823777341No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC72114.085066366041435No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC67103.8012474436469317No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT34741.968037797202599No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC15210.8616538542156457No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCCGATACT13430.7608159935645051No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT13230.7494858968621297No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACC11710.663377161924077No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC11060.6265543476413571No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT8710.4934257113884467No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT8460.4792630905104775No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC8310.47076551798369604No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC7690.4356422182063324No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCCCAGATACC6730.3812577540349307No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC5860.3319718333795979No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC5520.31271066898555977No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC5260.29798154327247184No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGATACT4580.25945921448439563No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC4490.2543606709683267No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC4350.24642960327666397No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC4290.24303057426595134No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC4020.22773494371774464No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC3330.18864611009454965No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCCGATACT3270.18524708108383706No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCACGATACT3210.18184805207312446No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGCTCCTTATTCTCACAGTACC3210.18184805207312446No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC3080.17448348921658047No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATAAT2820.15975436350349248No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTTACGGTACC2590.14672475229576085No Hit
TNACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC2570.1455917426255233No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTACC2370.13426164592314796No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGCTACT2330.1319956265826729No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTACTCTTACGGTACC2320.1314291217475541No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTACTCTCCCGATACT2310.13086261691243534No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCCCCCGATACT2300.1302961120773166No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCCCAGTACC2240.12689708306660397No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGAGCTTATTCTCCCGATACT2240.12689708306660397No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCTGATACT2150.12179853955053507No Hit
TTACCGCGGCTGCTGGCATGGAGTTAGCCGGAGCTTATTCTCCCGATACT2040.11556698636422862No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCCTATTCTCCCGATACT2030.11500048152910985No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTAAC2020.11443397669399108No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC2010.11386747185887232No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTATTCTCCCGATACT1960.11103494768327847No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGAGCTTATTCTCCCGATACT1880.10650290900232834No Hit
TACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACTG1830.10367038482673449No Hit
TTACCGCGGCTGCTGGCACGGGGTTAGCCGGAGCTTATTCTCCCGATACT1790.10140436548625943No Hit
TTACCGCCGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC1790.10140436548625943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)