FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185473
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG7409539.949210936362704No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG3130716.87954580990225No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG101155.453623977613993No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCTTAACACATG89854.8443708787802No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG68433.6894858011678253No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG60053.2376680163689593No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG46052.482841168256296No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG16530.8912348428073089No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG12380.6674825985453409No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG11270.607635612730694No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG9460.5100472845104139No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG7640.4119197942557677No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCCTAATACATG4590.24747537377408035No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCGTGCCTAACACATG4170.22483056833070042No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTAACACATG3980.21458648967774288No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCTTTACACATG3770.20326408695605291No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG3760.20272492492168673No Hit
AGAGTTTGATCCTGGCTCAGAACGAGCGCTGGCGGCATGCCTAATACATG3680.1984116286467572No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG3630.19571581847492628No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG3530.1903241981312644No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCATGCCTAATACATG3490.18816754999379964No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG2990.16120944827549025No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAATACATG2890.15581782793182836No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACGTG2680.14449542521013842No Hit
AGAGTTTGATCCTGGCTCGGAACGAACGCTGGCGGCATGCCTAATACATG2670.14395626317577223No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACACG2620.14126045300394127No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGTGTGCCTAATACATG2400.12939888824788512No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCACGCCTAATACATG2120.11430235128563188No Hit
AGAGTTTGATCCTGGCTCAGAACGGACGCTGGCGGCATGCCTAATACATG2110.11376318925126568No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG2010.1083715689076038No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCCGGCGGCATGCCTAATACATG1960.10567575873577287No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTCGCGGCAGGCTTAACACATG1900.10244078652957574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)