FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184877
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT5913831.987754020240484No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC1867210.099687900604186No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC93105.035780545984628No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC87644.74044905531786No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC43362.345343120020338No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC37292.0170167192241326No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC34821.883414378208214No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT32731.770366243502437No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC29911.617832396674546No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT16430.8886989728305846No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCCGATACT14840.8026958464276248No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACC13880.7507694304862151No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT11410.6171670894702964No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC8320.45002893815888395No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT7650.41378862703310854No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC7290.3943162210550798No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCGAGTAAC6700.36240311125775515No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC5730.30993579515028913No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGAGTAAC5440.2942496903346549No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGATACT5350.2893815888401478No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC5340.2888406886740914No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC5320.2877588883419787No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC5250.28397258717958423No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC4810.26017297987310484No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC4680.2531412777143722No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGATACC4190.22663716957761104No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCCGATACT3910.21149196492803324No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC3620.19580586011239903No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATAAT3380.18282425612704664No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC3260.1763334541343704No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC3150.17038355230775057No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCACGATACT3080.16659725114535612No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTACTCTCCCGATACT2770.14982934599760922No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTATTCTCCCGATACT2750.14874754566549653No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC2710.14658394500127112No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTGCGGGTACC2620.14171584350676397No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC2560.13847044251042584No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGAGCTTATTCTCCCGATACT2540.13738864217831315No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2500.13522504151408773No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCCCCCGATACT2330.12602973869112977No Hit
TNACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC2330.12602973869112977No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC2280.12332523786084804No Hit
TTACCGCGGCTGCTGGCACGGGGTTAGCCGGAGCTTATTCTCCCGATACT2270.12278433769479166No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGCTACT2260.12224343752873532No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCCTATTCTCCCGATACT2240.12116163719662262No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTTACGGTACC2170.11737533603422817No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATCCTCCCGATACT2140.1157526355360591No Hit
TTACCGCGGCTGCTGGCATGGAGTTAGCCGGAGCTTATTCTCCCGATACT2130.11521173537000276No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGACCCTTATTCATGAGTTACC2090.11304813470577736No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC2080.11250723453972099No Hit
TACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACTG2070.11196633437366464No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGATGCTTCCTCTGCTGGTACC1960.10601643254704478No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC1940.1049346322149321No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCAGGCCCTTATTCTTACGGTACC1890.10223013138465034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)