FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132684
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG4674935.23333634801483No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG1947614.678484218142355No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1518611.445238310572488No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG60994.596635615447228No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG43913.3093666154170807No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG26572.0025021856440866No Hit
AGAGTTTGATCCTGGCTCAGTGTGCCAGCAGCCGCGGTAAAGACCAAGTC23651.782430436224413No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG14541.0958367248500196No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG11750.8855626903017696No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG10600.7988905972084049No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG8570.6458955111392481No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG8570.6458955111392481No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG6350.47858068795031805No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG5450.4107503542250761No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCATAACACATG5050.3806035392360797No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG4200.3165415573844623No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAACACATG3830.2886557535196406No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCCTAACACATG3800.2863947423954659No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG3300.24871122365922038No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG3010.226854782792198No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG2830.21328871604714963No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGCGCCTAACACATG2830.21328871604714963No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG2760.20801302342407527No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGTGGCATGCTTAACACATG2740.20650568267462543No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2580.19444695667902687No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG2570.19369328630430196No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCTGCGTGCTTAACACATG2360.17786620843507883No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAACACATG2360.17786620843507883No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG2230.168068493563655No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG2150.16203913056585573No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG2140.1612854601911308No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATG1980.14922673419553223No Hit
AGAGTTTGATCCTGGCTCAGTGCCAGCAGCCGCGGTAAAGACCAAGTCTC1960.14771939344608243No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG1890.14244370082300806No Hit
AGAGTTTGATCCTGGCTCAGTGTGCCAGCAGCCGCGGTAGAGACCAAGTC1830.1379216785746586No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG1680.12661662295378492No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG1580.11907991920653584No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAATACATG1560.11757257845708602No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG1560.11757257845708602No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAGTACATG1390.10476018208676253No Hit
AGAGTTTGATCCTGGCTCAGGACGGACGCTGGCGGCGTGCCTAATACATG1370.10325284133731272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)