FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185437
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG3504318.897523148023318No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG3204517.28080156603051No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG2285312.323862012435491No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG184169.931135641754341No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG130467.035273435182838No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCTTAACACATG58313.144464157638444No Hit
AGAGTTTGATCCTGGCTCAGTGTGTGCCAGCAGCCGCGGTAAAGACCAAG31651.706779121750244No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATG29811.6075540480055222No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG28661.5455383769150708No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG20551.1081930790511063No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCTTAACACATG19811.068287342871164No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG18380.9911722040369506No Hit
AGAGTTTGATCCTGGCTCAGGATCAACGCTGGCGGCGTGCCTAATGCATG17830.9615125352545608No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG16500.8897900634716911No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG14530.7835545225602225No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGAATGCTTTACACATG9800.5284813710316711No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG7930.4276384971715461No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG7100.3828793606453944No Hit
AGAGTTTGATCCTGGCTCAGAACGAACACTGGCGGCATGCCTAACACATG7030.3791044937094539No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAACACATG5350.2885076872468817No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAACACATG2890.15584807778382953No Hit
AGAGTTTGATCCTGGCTCGGAACGAACGCTGGCGGCATGCCTAACACATG2670.14398421027087366No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCTTAACACATG2600.14020934333493315No Hit
AGAGTTTGATCCTGGCTCGGATTGAACGCTGGCGGCATGCCTTACACATG2490.13427740957845521No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTTACACATG2270.12241354206549934No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCATGCCTAACACATG2240.12079574195009626No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2240.12079574195009626No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCTTAACACATG2210.11917794183469317No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG2210.11917794183469317No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCCTAACACATG2140.11540307489875268No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG2040.11001040784740909No Hit
AGAGTTTGATCCTGGCTCAGTGTGTGCCAGCAGCCGCGGTAGAGACCAAG1930.10407847409093114No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG1890.10192140727039371No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)